Cellular Uptake
Cellular Uptake - Related Videos
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
05:25
Extracellular Vesicle Uptake Assay: A Method to Visualize and Quantify Cellular Uptake of Extracellular Vesicles Using 3D Fluorescence Imaging Technique
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2025
In this video, we demonstrate an assay to visualize and quantify the cellular uptake of extracellular vesicles or EVs. This assay utilizes 3D fluorescent imaging using a confocal microscope and allows for distinguishing internalized EVs from ones adhered to the cell surface.
05:52
In Vitro Glucose Depletion Assay: A Fluorescence-Based Method to Quantify Cellular Glucose Uptake via Extracellular Glucose Depletion Measurement
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2025
This video demonstrates an in vitro assay to indirectly measure intracellular glucose uptake. The assay uses a fluorescently-labeled glucose analog to establish a correlation between extracellular glucose depletion and intracellular glucose uptake.
07:13
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
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Cited by 1 •
2022
This article presents the integration of a spectral-focusing module and a dual-output pulse laser, enabling rapid hyperspectral imaging of gold nanoparticles and cancer cells. This work aims to demonstrate the details of multimodal nonlinear optical techniques on a standard laser scanning microscope.
06:28
Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Cited by 9 •
2015
The human lactoferrin (hLF) is a component of the immune system. In this study, immunofluorescence assays are used to demonstrate both the hepatocellular uptake of hLF and a qualitative reduction in the hepatitis C virus replication upon treatment with hLF.
FAQs
Membrane receptors help determine which extracellular material enters through receptor-mediated endocytosis. Their role makes uptake selective rather than indiscriminate, linking recognition at the cell surface to vesicle formation inside the cell. In immunology and infection research, this selectivity helps explain how cells capture particular foreign materials or interact with microorganisms.
Cytoskeletal remodeling and vesicle formation connect the cell surface to intracellular handling. Remodeling helps the membrane change shape as material is enclosed, while vesicles provide an internal compartment after entry. Examining both steps helps researchers distinguish failure of capture from altered intracellular processing, which can change immune or infection outcomes.
These routes can be compared by the type of control they emphasize. Receptor-mediated endocytosis uses membrane receptors, whereas phagocytosis is highlighted for capturing pathogens; pinocytosis is another named uptake route. Comparing them helps investigators relate entry behavior to nutrient acquisition, immune defense, or infection without treating all uptake events as equivalent.
During infection studies, investigators can ask whether a microorganism is being captured by a host-cell uptake pathway or using uptake to invade the cell. Connecting the entry route with receptor involvement, cytoskeletal remodeling, and vesicle formation can clarify host-pathogen interactions and identify where cellular handling may influence infection.
Antigen-presenting cells make uptake immunologically consequential by processing foreign material for immune recognition. Thus, studies should follow the connection between internalization and subsequent handling, rather than measuring entry alone. This perspective helps explain how extracellular material can contribute to immune defense and why altered uptake may affect recognition of infection.
Research on cellular uptake supports vaccine and drug-delivery design because it reveals how extracellular material enters cells and is subsequently handled. The same knowledge can guide efforts to modify host-pathogen interactions. In practical terms, uptake studies connect a delivery strategy or infectious process with the cell’s entry mechanisms and processing pathways.