Epithelial Ciliated Strips
Epithelial Ciliated Strips - Related Videos
08:42
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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Cited by 3 •
2016
Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.
11:46
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Cited by 29 •
2011
The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
01:13
Voltammetry: Stripping Methods
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2024
Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current. Anodic Stripping Voltammetry (ASV) ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
02:19
Determining the Antibacterial Efficacy of an Antibiotic-Loaded Polymeric Strip
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2025
Source: Sekar, A., et. al., A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials. J. Vis. Exp. (2023)This video demonstrates a luminescence-based assay for assessing the antibacterial efficacy of an antibiotic-loaded polymeric strip by quantifying ATP-driven luminescence, which decreases in bacterial cultures exposed to the antibiotic.
FAQs
Coordinated ciliary beating creates directional transport rather than random movement. Because mucus overlies the epithelial surface, particles and microbes trapped within it can move toward clearance sites. Transport behavior therefore provides a functional readout of mucosal defense: altered ciliary activity or coordination may indicate that an infectious agent or inflammatory signal is disrupting an early protective process.
Preserving the intact epithelial surface retains the spatial relationship between cilia, mucus, and the cells that interact with infectious agents. Researchers can therefore examine pathogen attachment or invasion in a tissue context while also monitoring epithelial damage. This connects a local interaction at the surface with changes in barrier-associated mucociliary protection.
Microbes and inflammatory signals can change ciliary activity, allowing investigators to assess how infection-related or immune-related conditions affect mucociliary transport. A change in beating provides more than a mechanical observation: it may reveal disruption of an early innate defense mechanism. Comparing ciliary behavior under different conditions helps connect epithelial responses with impaired or preserved clearance.
These preparations support separate examination of pathogen attachment, invasion, and epithelial damage. Attachment concerns interaction with the epithelial surface, invasion indicates penetration into the tissue, and damage reflects injury to the epithelial structure. Considering these processes together helps determine whether an infectious agent primarily remains at the surface, enters epithelial tissue, or compromises mucosal protection.
Investigators can use the strips to examine mucociliary transport alongside pathogen behavior and epithelial responses. Observations may focus on movement through the mucus layer, microbial attachment or invasion, epithelial damage, and changes in ciliary activity. This combined approach preserves relationships between physical clearance and tissue responses that would be difficult to interpret from a single outcome alone.
The preparation can provide information about how effectively mucus and trapped microbes move toward clearance sites, whether pathogens attach to or invade the epithelium, and whether the tissue shows damage. It can also reveal changes in ciliary activity associated with microbes or inflammatory signals. Together, these outcomes describe both mucosal function and infection-related disruption.
Mucociliary transport represents an early protective process that acts before later immune responses are fully engaged. Studying this process in epithelial ciliated strips helps connect physical removal of microbes with pathogen attachment, invasion, tissue damage, and altered ciliary activity. Those links provide context for understanding how epithelial dysfunction may contribute to respiratory disease outcomes.