Eps Detection
Eps Detection - Related Videos
02:50
An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection
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2025
This video demonstrates a fluorescence-based method for quantifying bile salt-induced biofilm formation. Upon the addition of bile salts in the culture of a pathogenic enteric bacteria, biofilm production is detected using a fluorescent lectin that binds to the polysaccharides in the extracellular polymeric substance (EPS) matrix of the biofilm.
01:21
EPS and iPS Cells in Disease Research
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2023
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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Cited by 10 •
2021
Here, we present an optimized on-filter digestion protocol with detailed information about the following: protein digestion, peptide purification and data independent acquisition analysis. This strategy is applied to the analysis of expressed prostatic secretions-urine samples and allows high proteome coverage and low missing value label-free profiling of the urinary proteome.
02:49
Detection of Biofilm Disassembly Through a Dispersion Assay
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2025
The video showcases a biofilm dispersion assay using a multi-well plate. Buffer and glucose treatment disassemble the biofilm, releasing bacteria, while bile salts induce stress, retaining bacteria in the protective extracellular polymeric substance. Higher dispersion with buffer and glucose confirms successful biofilm disassembly, estimated by colony forming units measurement.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
These methods separate matrix-associated signal from cellular signal by combining selective labeling with spatial or biochemical readouts. Matrix-binding stains and fluorescent labels mark EPS-related material, while microscopy shows whether the signal surrounds cells; biochemical measurements provide an abundance estimate. Using complementary readouts is valuable when researchers need to determine whether a change reflects matrix production rather than simply cell presence.
Because the matrix contains carbohydrates, proteins, and nucleic acids, no single measurement necessarily describes all of its material. Researchers can select a stain, fluorescent label, or biochemical assay according to the component or total abundance they want to compare. This distinction matters when linking matrix composition to antimicrobial penetration, immune-cell access, or differences in biofilm behavior.
Growth conditions are part of the measurement, not merely background information. EPS abundance can be estimated under defined conditions, allowing strains or treatments to be compared on a consistent basis. If conditions differ, an apparent change in signal may reflect altered growth context rather than the strain or intervention itself. Standardized conditions therefore strengthen interpretation of treatment-related differences.
A practical workflow begins by establishing microbial growth under defined conditions, followed by a matrix-focused assay. The sample can then be examined by microscopy or evaluated through a biochemical measurement, depending on whether spatial distribution or abundance is the priority. Comparing the resulting signals across strains or treatments creates a basis for assessing biofilm development and matrix-associated responses.
The result can indicate how much matrix-associated material is present and whether biofilm development changes across experimental groups. Microscopy adds information about where the labeled material appears relative to cells, whereas biochemical measurements emphasize quantity. Interpreting both dimensions helps connect EPS abundance with matrix organization and distinguishes a compositional change from a simple visual difference.
It helps investigators examine how biofilm matrix affects antimicrobial penetration and immune-cell access, two features relevant to microbial persistence and chronic infection. Measurements can also support comparisons of anti-biofilm strategies and strains, while relating matrix composition to host-pathogen interactions and disease-relevant outcomes. Thus, the assay provides a bridge between microbial structure and infection biology.