Hek-293t/17 Cells
Hek-293t/17 Cells - Related Videos
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Cited by 23 •
2011
Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).
09:29
Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems
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Cited by 1 •
2024
This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.
09:33
Rapid Isolation of the Mitoribosome from HEK Cells
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Cited by 25 •
2018
Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
12:38
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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2008
This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.
09:02
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Cited by 1 •
2020
This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).
FAQs
The SV40 large T antigen supports replication of plasmids that contain the SV40 origin. This feature can increase the availability of plasmid templates during transient transfection and thereby support strong gene expression. In practice, it helps investigators introduce genes, viral components, or immune factors efficiently when designing molecular assays and experimental infection models.
Their high transfection efficiency allows researchers to introduce genetic material into a large proportion of cells for short-term experiments. This is useful when an assay requires rapid expression of a recombinant protein, viral component, or immune factor without establishing a permanently modified cell population. The resulting platform supports reproducible gene-expression measurements and screening studies.
The growth characteristics of these cells make them suitable for producing recombinant viral particles after the relevant genetic components are introduced. Their ability to support efficient expression and maintain reproducible experimental performance helps researchers develop viral-vector production workflows. This role is distinct from using the cells only to measure gene activity or investigate responses to an infection-related factor.
They can serve as a production platform for pseudotyped viruses, which are viral particles engineered for experimental infection studies. These particles allow investigators to examine infection-related processes and gene delivery in a controlled laboratory system. In immunology and infection research, this application supports studies of host-pathogen interactions while using a defined system for testing viral entry or expression-related outcomes.
A typical use begins with transiently introducing the selected genetic material into the cells. The cells then provide a setting for gene expression or recombinant viral-particle production, depending on the experiment. Researchers can subsequently use the resulting expression system or particles in molecular assays, infection research, screening, or gene delivery to target cells.
These cells are useful when an experiment needs efficient gene expression, reproducible transient transfection, or a platform for generating viral particles. Investigators may apply them to study host-pathogen interactions, deliver genes or immune factors into target cells, or develop experimental models. Their value lies in enabling controlled molecular manipulation before examining infection-related or immune-associated outcomes.