Mammalian Cell Transfection
Mammalian Cell Transfection - Related Videos
02:06
Harvesting Adeno-Associated Virus from Transfected Mammalian Cells
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2026
Source: Rghei, A. D., et al. Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models. J. Vis. Exp. (2021)This video demonstrates the recovery of crude adeno-associated virus from transfected mammalian cells using chemical lysis and centrifugation, enabling high-yield AAV production for downstream purification and preclinical gene therapy applications.
02:29
Antibody Production from a Batch Overgrowth Culture of Transfected Mammalian Cells
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2025
The video demonstrates a method for producing therapeutic antibodies using stably transfected mammalian cells. Tryptone supplementation stimulates cell division, resulting in overgrowth and supporting a higher antibody yield. The antibody-containing medium is harvested, filtered, and stored for further use.
15:56
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
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Cited by 99 •
2009
We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.
15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
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Cited by 17 •
2010
Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.
07:28
An Introduction to Transfection
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2023
Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA into a cell enables the expression, or production, of proteins using the cells own machinery, whereas insertion of RNA into a cell is used to down-regulate the production of a specific protein by stopping translation. While the site of action for transfected RNA is the cytoplasm, DNA must be transported to the nucleus for effective transfection. There, the...
FAQs
Chemical transfection depends on electrostatic complex formation, whereas electroporation depends on temporary membrane permeabilization. Cationic lipids or polymers associate with negatively charged DNA or RNA and facilitate uptake into cells. Brief electrical pulses instead create a transient entry opportunity. This distinction helps researchers choose between a carrier-based mechanism and a physical delivery mechanism for a given experiment.
The choice between transient and stable transfection determines how long the introduced genetic material influences cells. Transient transfection supports short-term expression or gene suppression, whereas stable transfection is used when sustained alteration is needed. This distinction matters in immunology and infection studies because immediate signaling or antiviral responses may require different expression times than longer-term cellular models.
DNA and RNA reagents can support different approaches to altering gene expression. The system can express viral proteins, reporters, receptors, or immune mediators, while RNA-based reagents can reduce gene expression. Researchers can therefore use transfection either to add a measurable or functional signal or to decrease a selected gene output, enabling complementary ways to examine cellular function.
A practical workflow begins by choosing the desired outcome, then matching the nucleic acid and delivery mechanism to that goal. Chemical approaches require forming complexes between nucleic acids and cationic lipids or polymers before cellular uptake, while electroporation requires brief electrical pulses. The design should also distinguish short-term from sustained expression, depending on whether transient or stable transfection is selected.
By introducing viral proteins, receptors, reporters, or immune mediators, researchers can manipulate selected components of mammalian cells and examine consequences for host-pathogen biology. RNA-based reagents can reduce gene expression, allowing researchers to test how diminished expression affects cellular behavior. These options help connect a candidate host or viral factor with signaling behavior, antiviral responses, or other infection-related cellular functions.
Transfected cells can be configured to express viral proteins, immune mediators, receptors, or reporters, creating controlled systems for examining cellular responses. Researchers can then study host-pathogen interactions, antiviral responses, and signaling pathways, or evaluate candidate therapeutics in relation to those processes. The resulting model links a chosen genetic manipulation with an observable cellular function relevant to infection research.