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MISSION esiRNA for RNAi Screening in Mammalian Cells
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MISSION esiRNA for RNAi Screening in Mammalian Cells

Article DOI: 10.3791/2008 • 15:32 min • May 12th, 2010
May 12th, 2010 •
1, 1
1Max Planck Institute of Molecular Cell Biology and Genetics

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Here we use a human esiRNA library in a high-throughput screen for genes involved in cell division. We demonstrate how to set up and conduct an esiRNA screens, as well as how to analyze and validate the results.

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MISSION EsiRNA RNAi Screening Mammalian Cells Gene Expression Control Small Interfering RNAs (siRNAs) Dicer RNA-induced Silencing Complex (RISC) Target MRNA Cleavage Loss Of Gene Function Studies Chemical Synthesis Of SiRNAs Endonucleolytic Cleavage Target-specific Long Double-stranded RNAs RNase III Endoribonuclease-prepared SiRNA Efficacy Of SiRNAs And EsiRNAs Off-target Effects
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