Pbmc Cryopreservation
Pbmc Cryopreservation - Related Videos
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
02:26
A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model
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2025
This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T cells.
11:10
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
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Cited by 23 •
2011
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
FAQs
Controlled-rate cooling reduces the likelihood of intracellular ice formation as the sample reaches very low temperatures. This is important because ice inside cells can damage cellular structures and reduce recovery quality. Managing the cooling process therefore supports later preservation of PBMC viability and function, which is essential when samples will undergo immunological analysis after storage.
DMSO serves as a cryoprotective component in the suspension medium. Its use supports protection during cooling, while the overall freezing strategy limits intracellular ice formation. Because retained cryoprotectant can contribute to osmotic or chemical injury during recovery, dilution or washing after thawing becomes an important part of handling the cells safely.
These steps address different risks at different stages. Controlled cooling helps limit intracellular ice formation during storage preparation, whereas rapid thawing helps reduce injury as cells return from very low temperatures. Using both approaches supports better preservation of cell viability and function than treating freezing and recovery as separate, unmanaged events.
Recovery generally includes rapid thawing, followed by dilution or washing to reduce exposure to the cryoprotective medium. Careful handling during these steps helps limit osmotic and cryoprotectant-related injury. After recovery, the preserved cells can be used for immunological analysis, provided their viability and function remain suitable for the intended assay or study.
Preserved PBMCs provide material for standardized immune assays and pathogen-response studies. They can also support biobanking, allowing samples collected at different times to remain available for later analysis. This flexibility helps investigators examine immune responses to infection and perform planned comparisons without requiring every analysis to occur immediately after sample collection.
Cryopreservation makes samples from different time points or cohorts available for analysis under a more consistent study workflow. Rather than relying only on immediate testing, investigators can draw on stored material for later immunological comparisons. This supports organized biobanking and helps extend the value of samples used in immune and infection studies.