Ros Detection Assay
Ros Detection Assay - Related Videos
03:50
ROS Detection with DCFH-DA Staining: Measuring Total ROS in Colorectal Cancer Cell Lines
0 Views •
2023
This video describes the protocol for detecting total cellular reactive oxygen species (ROS) using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). This method helps visualize ROS localization in the adherent cells using a fluorescence microscope and further quantify ROS intensity with a fluorescence plate reader.
07:17
Production and Detection of Reactive Oxygen Species (ROS) in Cancers
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Cited by 278 •
2011
Here we propose simple methods to test and evaluate the presence of reactive oxygen species in cells.
07:33
Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Cited by 10 •
2011
An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.
03:47
Determination of ROS Production by Neutrophils upon Interaction with Opsonized Biofilm
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2025
In this video, we demonstrate an in vitro chemiluminescence assay to detect the generation of reactive oxygen species by neutrophils upon exposure to an opsonized bacterial biofilm.
03:42
An Assay for Detecting Reactive Oxygen Species Production in Rice Tissues upon Immune Elicitation
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2025
This video demonstrates an assay for real-time detection of reactive oxygen species (ROS) production in rice tissues upon pathogen-associated molecular pattern (PAMP) elicitation. Cut sheath segments and leaf discs are exposed to a bacterial flagella-derived peptide functioning as a PAMP. Upon recognition of the peptide via pattern recognition receptors (PRRs) on plant cells, a rapid and transient production of ROS occurs, which is detected via a chemiluminescence assay.
FAQs
A redox-sensitive probe reacts with reactive oxygen species through oxidation, causing a change in fluorescence or absorbance. The measured signal therefore serves as an indicator of ROS generation under the selected experimental conditions. Comparing that signal with appropriate controls helps distinguish treatment-associated changes from baseline or non-specific changes in the sample.
ROS production can vary with the biological stimulus and experimental setting, so signal intensity has meaning only relative to the conditions in which it was measured. Controls provide the comparison needed to evaluate whether a change reflects altered ROS generation. This is especially important when assessing immune-cell activation, pathogen exposure, or inflammatory responses.
Both fluorescence and absorbance provide measurable readouts after probe oxidation, but the assay format determines how the response is recorded. A change in either signal can be compared across defined conditions to evaluate relative ROS generation. These measurements support analysis of oxidative bursts, pathogen-triggered responses, and oxidative stress linked to tissue injury.
A typical workflow places a redox-sensitive probe in cells or another sample, exposes the preparation to defined experimental conditions, and records the resulting fluorescence or absorbance. The signal is then compared with appropriate controls to assess ROS generation. This sequence allows investigators to examine how an immune stimulus, infection-related condition, or other treatment changes the oxidative response.
Investigators can apply the assay when they need to quantify oxidative bursts in immune cells or evaluate responses triggered by pathogens. The resulting measurements help compare ROS generation under different infection-related conditions. They can also support studies of antimicrobial mechanisms by examining how oxidative responses relate to microbial survival or to damage associated with inflammation.
In immunology, these assays connect redox activity with host defense and inflammation by measuring changes associated with immune-cell responses. Results can help characterize antimicrobial mechanisms and examine how oxidative stress may contribute to inflammatory disease or tissue injury. Because interpretation depends on controls and defined conditions, the assay supports comparative analysis rather than an isolated signal alone.