Tonsil Cell Isolation
Tonsil Cell Isolation - Related Videos
08:09
Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
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Cited by 11 •
2015
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.
01:30
Tonsillitis I: Introduction
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2024
Tonsillitis is inflammation of the tonsils, which are two lymphoid tissue masses at the back of the throat. This condition can cause discomfort and irritation in the throat. Etiology Three primary contributing factors have been identified. • Viruses: Viral infections are the most common cause of tonsillitis. Common viruses responsible for tonsillitis include rhinovirus, influenza, parainfluenza, Epstein-Barr virus, and adenovirus. • Bacteria: The predominant bacterium responsible for...
01:26
Tonsillitis II: Management
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2024
This lesson will focus on the different treatment options for managing tonsillitis, which typically depend on the cause and severity. • Acute viral tonsillitis treatment usually involves outpatient supportive management, including analgesia (acetaminophen) and oral hydration, corticosteroids (dexamethasone), salt-water gargles, and rest. • Bacterial tonsillitis is typically treated with penicillin as the primary choice, although cephalosporins can also be effective. Antibiotic therapy with...
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
FAQs
Mechanical dissociation separates the tissue into a cell suspension, while optional enzymatic digestion can provide an additional means of releasing cells from the tissue. These approaches must be balanced against the need to preserve viable immune and epithelial cells. The resulting preparation can therefore support direct cellular characterization while retaining relevance to local tonsil immune responses.
Prompt, gentle handling helps maintain cell viability after tissue collection and during processing. Appropriate buffered conditions support the cells while mechanical disruption, filtration, and centrifugation remove unwanted material. If processing is delayed or unnecessarily harsh, the recovered suspension may contain fewer viable cells, limiting its suitability for culture, flow cytometry, or stimulation experiments.
The preparation can contain immune and epithelial cells that contribute different perspectives on local host defense. Researchers can examine lymphocyte activation, antigen presentation, and cytokine production, then relate those responses to exposure to pathogens or immune stimuli. This makes the model useful for connecting cellular behavior with infection-related inflammation in tonsil tissue.
A typical workflow mechanically dissociates the tissue, with enzymatic digestion included when appropriate, and produces a cell suspension. Filtration removes larger debris, followed by centrifugation to further clear the preparation and enrich cells. Because downstream analyses depend on viable material, the workflow emphasizes prompt processing, gentle manipulation, and suitable buffered conditions throughout.
After recovery, the cells can be characterized by flow cytometry to assess the populations present and their response-associated features. They may also be placed in culture or exposed to pathogens and immune stimuli. These options allow investigators to study cellular composition, activation, antigen presentation, cytokine production, and infection-related responses using the same tissue-derived preparation.
Tonsil-derived cells provide a way to investigate immune activity at a mucosal site rather than relying only on generalized immune models. Their responses can inform studies of mucosal immunity, host-pathogen interactions, and inflammation. The approach also supports translational immunology by enabling direct analysis of human immune and epithelial cell behavior from tonsil tissue.