Ligature Holder
Ligature Holder - Related Videos
08:22
Permanent Cerebral Vessel Occlusion via Double Ligature and Transection
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Cited by 16 •
2013
We describe a highly reproducible method for the permanent occlusion of a rodent major cerebral blood vessel. This technique can be accomplished with very little peripheral damage, minimal blood loss, a high rate of long-term survival, and consistent infarct volume commensurate with the human clinical population.
07:15
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
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Cited by 4 •
2020
This article presents a protocol for establishing a ligature-induced model of murine periodontitis involving multiple maxillary molars, resulting in larger areas of the involved gingival tissue and bone for subsequent analysis as well as reduced animal usage. A technique to assess oral neutrophils in a manner analogous to human subjects is also described.
07:55
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
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Cited by 4 •
2019
A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.
05:41
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
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Cited by 2 •
2022
The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.
05:37
Experimental Model of Ligature-Induced Peri-Implantitis in Mice
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Cited by 5 •
2024
This is a report on an experimental model of ligature-induced peri-implantitis in mice. We describe all surgical steps, from pre- and post-operative management of the animals, extractions, implant placement, and ligature-induced peri-implantitis.
FAQs
Positioning near the gingival margin creates a localized, plaque-retentive site rather than a uniformly affected oral environment. Retained material supports microbial accumulation at a defined anatomical location, which can stimulate nearby tissue inflammation. This localization helps investigators relate changes in the host response to a specific periodontal site.
The fine tip supports controlled grasping, guiding, and securing of the ligature around a small anatomical structure. That control matters because placement must remain near the gingival margin without unnecessary tissue injury. Consistent positioning makes differences in microbial accumulation, inflammation, or bone loss more interpretable across experimental animals or study groups.
In this model, the ligature creates a site where plaque can accumulate, linking a localized microbial condition to nearby host inflammation. Investigators can then examine how immune responses accompany periodontal infection and inflammatory bone loss. The approach therefore connects microbial retention with tissue outcomes in a controlled anatomical setting.
A basic workflow is to grasp the ligature with the fine tip, guide it around the selected small anatomical structure, and secure it near the gingival margin. Placement should be controlled and consistent between subjects. Careful manipulation helps maintain the intended plaque-retentive condition while limiting unnecessary tissue injury.
The resulting localized condition can support assessment of host immune responses, periodontal infection, and inflammatory bone loss. Because the ligature remains positioned near the gingival margin, investigators can study these outcomes in relation to a defined plaque-retentive site. Consistent placement also improves comparison among experimental conditions.
Careful handling serves both scientific and procedural purposes. Reproducible placement helps ensure that observed inflammation or bone loss reflects the intended ligature-associated condition rather than inconsistent positioning. Limiting unintended contamination further supports interpretation of infection-related findings, while reducing unnecessary tissue injury helps maintain a controlled and responsible animal-model procedure.