3d Mps Platform
3d Mps Platform - Related Videos
10:01
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
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Cited by 20 •
2012
We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.
07:42
A High-Throughput Platform for Culture and 3D Imaging of Organoids
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Cited by 4 •
2022
This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.
10:42
Developing 3D Organized Human Cardiac Tissue within a Microfluidic Platform
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Cited by 4 •
2021
The goal of this protocol is to explain and demonstrate the development of a three-dimensional (3D) microfluidic model of highly aligned human cardiac tissue, composed of stem cell-derived cardiomyocytes co-cultured with cardiac fibroblasts (CFs) within a biomimetic, collagen-based hydrogel, for applications in cardiac tissue engineering, drug screening, and disease modeling.
10:12
Three Dimensional Vestibular Ocular Reflex Testing Using a Six Degrees of Freedom Motion Platform
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Cited by 4 •
2013
A method is described to measure three-dimensional vestibulo ocular reflexes (3D VOR) in humans using a six degrees of freedom (6DF) motion simulator. The gain and misalignment of the 3D angular VOR provide a direct measure of the quality of vestibular function. Representative data on healthy subjects are...
08:10
Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
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Cited by 5 •
2019
The cardiac extracellular matrix (ECM) is a complex network of molecules that orchestrate key processes in tissues and organs while enduring physiological remodeling throughout life. Standardized decellularization of fetal and adult hearts permits comparative experimental studies of both tissues in a 3D context by capturing native architecture and biomechanical properties.
FAQs
Organizing cells in three dimensions preserves relationships that are difficult to represent in flat cultures. Cells can occupy different positions, interact with neighboring cell types, and receive localized biochemical signals within a scaffold, matrix, or device. This arrangement helps researchers examine how tissue organization influences function and disease mechanisms under more physiologically relevant conditions.
Fluid flow provides a controlled physical and biochemical environment within microfluidic systems. It can shape how cells encounter nutrients, signals, and test compounds while contributing to tissue-level conditions that are not reproduced by static culture alone. Incorporating flow therefore helps investigators evaluate cellular responses in models designed to reflect important features of human physiology.
Two-dimensional cultures place cells on a flat surface, whereas three-dimensional systems preserve spatial organization, matrix or scaffold interactions, and cell-to-cell relationships. These differences can alter how cells function and respond to biochemical signals or treatments. Comparing the approaches helps researchers judge whether a result depends on simplified culture conditions or better reflects tissue-like behavior.
The outcome depends on how engineered human cells or tissues are arranged and how the platform controls their surrounding environment. Scaffold or matrix composition, spatial architecture, biochemical signaling, cell interactions, and fluid flow each contribute to the modeled physiology. Adjusting these features allows researchers to investigate different disease mechanisms or treatment responses within a controlled laboratory setting.
A basic setup begins by selecting engineered human cells or tissues relevant to the question, then organizing them within a three-dimensional scaffold, matrix, or microfluidic device. Researchers establish controlled biochemical and fluid conditions before examining tissue function, disease-related behavior, or responses to treatment. The resulting system can then support structured comparisons across experimental conditions.
Researchers may select this approach when tissue architecture, cell interactions, biochemical signaling, or fluid flow are important to the question. It is particularly relevant for studying disease mechanisms, evaluating drug efficacy, and assessing toxicity in a setting that can be more physiologically relevant than many conventional two-dimensional cultures. The platform can strengthen preclinical investigation before therapies advance.
Because 3D MPS platforms use engineered human cells and tissues, they can be configured to examine how particular biological systems respond to treatment. Researchers can study differences in treatment responses alongside drug efficacy and toxicity, supporting evaluation of personalized responses. These results may help guide development of therapies that are safer and more effective for human use.