Dorsal Skinfold Chamber
Dorsal Skinfold Chamber - Related Videos
08:52
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
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Cited by 32 •
2018
This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.
09:34
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
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Cited by 15 •
2019
Here, we present a protocol for an in vitro scratch assay using primary fibroblasts and for an in vivo skin wound healing assay in mice. Both assays are straightforward methods to assess in vitro and in vivo wound healing.
10:25
A Dorsal Skinfold Window Chamber Tumor Mouse Model for Combined Intravital Microscopy and Magnetic Resonance Imaging in Translational Cancer Research
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Cited by 4 •
2024
Translation of Intravital microscopy findings is challenged by its shallow depth penetration into tissue. Here we describe a dorsal window chamber mouse model that enables co-registration of intravital microscopy and clinically applicable imaging modalities (e.g., CT, MRI) for direct spatial correlation, potentially streamlining clinical translation of intravital microscopy findings.
10:24
Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
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Cited by 15 •
2014
The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.
09:48
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration
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Cited by 9 •
2017
This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.
FAQs
The chamber allows the same living tissue to be examined repeatedly over time rather than assessed only after separate tissue samples are collected. This longitudinal design lets investigators follow evolving microcirculation, inflammation, wound healing, or tumor growth within an individual small-animal model. It can therefore reveal how vascular and cellular changes develop alongside a treatment response.
The glass coverslip creates an optical access point to exposed, vascularized tissue while maintaining the preparation as a sealed chamber. Intravital microscopy can then be used to observe living tissue directly, including microcirculatory behavior and vascular permeability. This access connects visible changes in the tissue and its vessels with processes occurring during disease progression, repair, or treatment.
The exposed vascularized tissue provides the biological site in which microcirculation, permeability, inflammation, healing, and tumor-related changes can be followed. Because these features are observed in living tissue, investigators can examine vascular and tissue responses together rather than treating them as isolated measurements. That relationship is especially relevant when evaluating disease mechanisms or therapeutic compounds.
Repeated tissue sampling generally requires obtaining new material at multiple time points, whereas the chamber supports repeated, noninvasive observation of the same implanted preparation. This distinction reduces the need for serial tissue collection and preserves the ability to track changes over time. As a result, treatment effects can be related to an evolving biological response rather than to disconnected observations.
The model is suited to disease-mechanism studies because it permits microcirculation, vascular permeability, inflammation, wound healing, and tumor growth to be followed in living tissue. Investigators can observe these processes repeatedly and examine how they change together over time. This makes the chamber useful when the research question concerns progression and interaction among vascular and tissue responses.
For therapeutic-compound studies, observations can be paired with treatment outcomes rather than limited to a final tissue assessment. Researchers can examine how cellular and vascular changes accompany the response to a compound in the monitored tissue. This supports investigations of drug delivery and treatment effects while reducing the need for repeated tissue sampling in small-animal models.
Linking cellular and vascular changes with treatment outcomes helps investigators interpret whether a therapy is associated with coordinated changes in living tissue. Rather than recording a single endpoint, they can relate microcirculatory or permeability findings to broader responses such as inflammation, healing, or tumor growth. This integrated view supports medical research on mechanisms, drug delivery, and tissue repair.