Eus-bd
Eus-bd - Related Videos
10:53
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
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Cited by 37 •
2014
Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.
13:46
An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
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Cited by 4 •
2020
Recombinant BDNF containing an Avi sequence (BDNFAvi) is produced in HEK293 cells in a cost-effective manner and is purified by affinity chromatography. BDNFavi is then directly mono-biotinylated with the enzyme BirA in a tube. BDNFavi and mono-biotinylated BDNFavi retain their biological activity when compared to commercially available BDNF.
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
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2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
09:46
Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus
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Cited by 9 •
2015
The mechanism underlying the therapeutic effects of Deep Brain Stimulation (DBS) surgery needs investigation. The methods presented in this manuscript describe an experimental approach to examine the cellular events triggered by DBS by analyzing the gene expression profile of candidate genes that can facilitate neurogenesis post DBS surgery.
07:51
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
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Cited by 5 •
2016
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
FAQs
Ultrasound guidance allows the echoendoscope to identify the obstructed bile duct or gallbladder from within the gastrointestinal tract before access is created. This visualization directs the puncture site and helps establish a route for guidewire advancement. The approach links imaging with intervention, supporting targeted biliary drainage when standard access is unavailable or unsuccessful.
EUS-BD provides an alternative when conventional endoscopic drainage cannot be completed or is not feasible. It creates access through the gastrointestinal wall, whereas percutaneous drainage is identified as a separate drainage option in the clinical context. This distinction gives clinicians another internal drainage strategy for selected patients with biliary obstruction.
After the target has been punctured, the guidewire provides support for introducing the stent along the intended route. The stent then maintains the bypass pathway so bile can flow internally beyond the obstruction. Together, these components convert ultrasound-identified access into a sustained drainage route rather than a temporary puncture.
The procedure begins with echoendoscopic ultrasound identification of either an obstructed bile duct or the gallbladder. The selected structure is punctured through the gastrointestinal wall, and a guidewire is passed to support stent placement. Once positioned, the stent establishes the internal route intended to restore bile drainage around the blockage.
Clinicians may consider EUS-BD when conventional endoscopic drainage has failed or cannot be used. The technique is relevant for selected patients with biliary obstruction, including those with malignant disease. Its role is especially important when restoring internal bile flow could help address obstruction-related jaundice and associated symptoms.
By bypassing an obstructed segment, EUS-BD can restore bile drainage and may relieve jaundice and related symptoms. In medicine, it expands therapeutic options within advanced endoscopy and offers an alternative to percutaneous drainage for appropriate patients. Its value therefore lies in both symptom management and broader procedural choice for complex biliary obstruction.