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JoVE Journal
Neuroscience

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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
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Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Article DOI: 10.3791/51899
September 15th, 2014 •
1, 2, 1, 3,4, 1, 1
1Department of Neurosciences, University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 3Department of Anesthesiology, University of California, San Diego, 4VA San Diego Healthcare System

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Summary
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September 15th, 2014

Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.

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