Intraluminal Filament Model
Intraluminal Filament Model - Related Videos
06:28
Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
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Cited by 194 •
2011
Filamentous occlusion of the Middle cerebral artery is a common model for studying ischemic stroke in mice.
09:40
Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
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Cited by 97 •
2012
The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.
13:01
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
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Cited by 76 •
2011
The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.
05:53
Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium
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Cited by 5 •
2016
After puncturing the aorta through the inferior vena cava (IVC) to create an aorto-caval fistula in the mouse, solution containing a drug is infused into the IVC via the same needle, followed by incubation. This method enables more robust drug delivery to the venous endothelium compared to the external route.
08:58
Filamentous Fungi
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Fungi are heterotrophic eukaryotic organisms, and with the exception of yeasts, are aerobic. They are abundant in surface soils and are important for their role in nutrient cycling and the decomposition of organic matter and organic contaminants. White rot fungi (phanerochaete chryosporium) for example, (Figure 1) are known to degrade aromatics. Figure 1. White rot on...
FAQs
Reperfusion allows investigators to examine injury caused not only by interrupted circulation but also by the transition to restored blood flow. This reversible sequence helps distinguish effects associated with ischemia from those emerging after circulation returns. As a result, the model supports studies of ischemia-reperfusion injury and of interventions intended to protect brain tissue during or after reperfusion.
The model can be used to assess infarct development, neurological deficits, inflammatory responses, and tissue damage. These outcomes provide complementary information: infarcts indicate the extent of affected brain tissue, neurological findings reflect functional impairment, and inflammatory or structural changes help characterize the biological response. Together, they support evaluation of how cerebral ischemia progresses and recovers.
Its controlled and reversible design is particularly valuable because researchers can produce a focal ischemic event and then permit reperfusion within the same experimental framework. The approach therefore offers a consistent way to investigate stroke-related injury while examining both restricted circulation and restored circulation. This combination makes it useful for preclinical studies in cerebrovascular medicine.
The procedure consists of introducing a coated filament through the carotid artery, advancing it into the middle cerebral artery, and maintaining the resulting blockage for the experimental ischemic period. Withdrawal of the filament then permits reperfusion. This sequence creates the paired ischemia and reperfusion phases needed to study both stages of the injury process.
Researchers may use it when they need to test whether a candidate neuroprotective treatment limits damage associated with focal cerebral ischemia, reperfusion, or both. Treatment effects can be examined through infarct development, neurological deficits, inflammatory responses, and tissue damage. The controlled, reversible setup also supports comparison of outcomes under defined experimental conditions.
In medicine, the model provides a preclinical platform for investigating stroke mechanisms and assessing reperfusion strategies before they are considered in broader research programs. Because it reproduces a focal vascular event followed by restored circulation, investigators can relate structural injury, functional deficits, inflammation, and tissue damage to potential therapeutic effects in cerebrovascular disease.