Lucifer Yellow Permeability
Lucifer Yellow Permeability - Related Videos
09:48
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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Cited by 2 •
2022
The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.
06:22
Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
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Cited by 26 •
2019
We present a fluorescence assay to demonstrate that Lucifer Yellow (LY) is a robust marker to determine the apparent paracellular permeability of hCMEC/D3 cell monolayers, an in vitro model of the human blood-brain barrier. We used this assay to determine the kinetics of a confluent monolayer formation in cultured hCMEC/D3 cells.
01:18
Yellow Fever
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2026
Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...
02:01
Assessing Pseudovirus Infection Using Luciferase Reporter Assay
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2026
Source: Chang, X., et al. Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity. J. Vis. Exp. (2021).This video demonstrates a luciferase-based assay to quantify pseudovirus infection in epithelial cells. The pseudovirus encodes the luciferase gene, which is expressed in infected cells. After washing, and cells are incubated with lysis buffer at an ultra-low temperature, and then thawed to induce lysis.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
FAQs
Its low passive permeability makes Lucifer Yellow a useful probe for detecting barrier defects rather than ordinary movement through intact lipid membranes. When a monolayer becomes less restrictive, dye passage can rise, producing a fluorescence-based readout of altered barrier integrity. This contrast helps investigators interpret permeability changes as evidence of compromised cellular boundaries or enhanced paracellular transport.
Interpretation depends on the amount of dye passage relative to the barrier condition being examined. Greater Lucifer Yellow movement is consistent with loosened cell junctions or barrier injury, whereas limited movement supports preserved restriction. The assay therefore provides a comparative indicator of integrity, not merely a fluorescence measurement considered in isolation.
An epithelial monolayer can model epithelial barrier behavior, while an endothelial monolayer supports evaluation of vascular barrier function. Using the appropriate cultured cell layer connects the fluorescence result to the biological barrier under study. This choice allows permeability changes to be examined in a medicine-relevant tissue context.
A basic assay workflow exposes a cultured epithelial or endothelial monolayer to Lucifer Yellow and then assesses fluorescence associated with dye passage across the barrier. Investigators can compare that signal between barrier conditions, such as before and after a treatment or injury model. The resulting comparison indicates whether permeability has increased or remained restricted.
To examine a drug or disease-related injury, investigators compare Lucifer Yellow passage under the relevant experimental conditions. An increase in fluorescence-based permeability can indicate that the intervention or injury has weakened barrier integrity, whereas less passage suggests retained restriction. This design helps assess how the intervention or injury changes barrier function.
In drug-delivery studies, Lucifer Yellow permeability can serve as a barrier-function readout alongside the question of whether a delivery strategy changes passage. In tissue models, the same measurement helps evaluate whether the model maintains cellular integrity. These applications make the assay useful for linking experimental conditions with barrier performance in biomedical research.