Perfusion Analysis Method
Perfusion Analysis Method - Related Videos
06:05
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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Cited by 3 •
2017
Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.
10:37
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System
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Cited by 3 •
2022
A convenient gravity-fed perfusion method for histological analysis of the mouse central nervous system is presented. Immunofluorescent detection of phosphorylated α-synuclein is demonstrated in a mouse model of Parkinson's disease. This work also comprehensively describes the transcardial perfusion, dissection, tissue freezing/embedding, and frozen sectioning steps.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
08:33
An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
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Cited by 4 •
2021
We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
FAQs
The passage of a tracer, contrast agent, or physiological signal through tissue creates a changing signal over time. An analysis method interprets that time course to estimate distinct features, including blood volume, flow, transit time, or tissue enhancement. These measures describe complementary aspects of microcirculatory delivery rather than a single, interchangeable indicator of tissue perfusion.
Each provides a measurable marker of how blood moves through the microcirculation. The analysis tracks the marker as it reaches and leaves tissue, then relates its changing signal to perfusion parameters. Depending on the study, this marker may be an introduced tracer or contrast agent, or a naturally occurring physiological signal used to characterize tissue enhancement and delivery.
Structural imaging may show the shape or appearance of an organ without directly indicating whether its tissue receives sufficient blood supply. Perfusion measurements add functional information by identifying altered flow, blood volume, transit time, or enhancement. This distinction is clinically important because ischemia or impaired vascular function may be detectable through circulation changes before obvious anatomical abnormalities appear.
A typical workflow begins by selecting a measurable tracer, contrast agent, or physiological signal and acquiring observations as it passes through tissue. The resulting time-dependent data are then analyzed to quantify parameters such as flow, blood volume, transit time, or enhancement. Interpretation links those measurements to the condition of the examined organ or tissue.
In medicine, these methods can be applied to cerebral, cardiac, and renal tissue, as well as tumors. They help assess whether circulation is adequate and can reveal ischemia or impaired vascular function. In oncology-related evaluation, perfusion measurements may also show treatment-related changes, adding functional evidence to the assessment of tissue response.
Standardized analysis makes measurements more consistent when clinicians compare tissues, examinations, or changes over time. Consistent quantification of flow, blood volume, transit time, or enhancement can support diagnosis, treatment planning, and monitoring of disease progression. It also helps identify treatment-related changes that may not be apparent from anatomical assessment alone.