Recipient Mouse Preparation
Recipient Mouse Preparation - Related Videos
12:52
Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA First discovered by Lederberg and Tatum in 1946, conjugation is a form of horizontal gene transfer between bacteria that relies on direct physical contact between two bacterial cells (1). Unlike other forms of gene transfer, such as transformation or transduction, conjugation is a naturally occurring process in which...
11:21
Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA Transduction is a form of genetic exchange between bacteria that utilizes bacteriophages, or phages, a class of virus that infects exclusively prokaryotic organisms. This form of DNA transfer, from one bacterium to another by way of a phage, was discovered in 1951 by Norton Zinder and Joshua Ledererg, who termed the...
03:15
A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients
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2025
This video demonstrates an assay to detect anti-donor HLA alloantibodies in organ recipients. A peptide array is synthesized to represent potential epitopes for antibody-mediated organ rejection. The array is incubated with the recipient's serum, containing alloantibodies that bind to their target epitopes. Antibody binding is detected using chemiluminescence to identify donor-specific HLA epitopes that incite alloantibody reactivity.
04:05
Subcutaneous Transplantation Assay: A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model
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2023
This video describes the method for transplantation of tumor cells from the melanoma-bearing donor zebrafish model to an immunocompromised recipient Casper zebrafish. It helps to visualize tumor engraftment and proliferation in the recipient fish.
10:01
En Face Preparation of Mouse Blood Vessels
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Cited by 20 •
2017
A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.
FAQs
Conditioning reduces endogenous immune or hematopoietic activity before transplantation. This creates a more receptive biological setting for donor cells, tissues, or organs and can improve engraftment, meaning the donor material becomes established in the recipient. Irradiation and immunosuppressive treatment are examples of conditioning approaches used for this purpose.
Both approaches serve the central preparation goal of reducing activity that could interfere with donor material after transplantation. Irradiation and immunosuppressive treatment represent different ways to condition the recipient, allowing investigators to establish transplantation models in which donor-cell establishment, immune reconstitution, or graft responses can be evaluated.
Monitoring and supportive care help maintain the animal during the conditioning period, when reduced immune or hematopoietic activity may make consistent study management especially important. These measures also support standardized preparation across experiments, improving the reliability of subsequent assessments of engraftment, graft rejection, treatment safety, and therapeutic outcomes.
Standardization limits variation in the recipient state before donor material is introduced. Consistent conditioning and care make differences in engraftment, immune reconstitution, graft rejection, or graft-versus-host disease easier to interpret as study outcomes rather than consequences of uneven preparation. This is particularly important when evaluating compatibility or treatment effects.
The process includes applying an appropriate conditioning approach, maintaining the mouse with monitoring and supportive care, and then proceeding with transplantation of the intended donor cells, tissue, or organ. The preparation must remain consistent with the study objective, because the resulting recipient state influences interpretation of engraftment and immune-related outcomes.
Researchers use it in studies involving hematopoietic stem-cell transplantation, immune reconstitution, graft rejection, and graft-versus-host disease. The same preparation framework also supports investigations of transplant compatibility, treatment safety, and therapeutic outcomes. Its value comes from creating a controlled recipient model in which donor material and post-transplant responses can be examined.
These models can support evaluation of whether donor material becomes established, how immune function is reconstituted, and whether rejection or graft-versus-host disease develops. They can also reveal how compatible a transplant is and whether a treatment appears safe or therapeutically beneficial, connecting preparation procedures to clinically relevant transplantation questions.