Single Fiber Electrophysiology
Single Fiber Electrophysiology - Related Videos
12:53
Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
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Cited by 17 •
2011
The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber interneurons.
03:55
In Vivo Single-Fiber Recording of Sciatic Nerve C-Fibers in Rats
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2025
This video demonstrates a single-fiber recording of C-fiber nerve conduction in rats. An anesthetized rat is incised to expose the sciatic nerve, which provides sensory and motor supply to the lower limbs. The individual C-fibers are then exposed. Electrical stimulation is provided using stimulus electrodes implanted in the foot, and a response is recorded from a single nerve fiber.
09:39
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
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Cited by 6 •
2012
A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.
08:19
In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats
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2017
Abnormal sensory function underlies visceral pain and other symptoms of functional and inflammatory bowel diseases. A protocol for the electrophysiological recording of the colonic afferent nerves in an ex vivo rat colorectum preparation is presented here.
09:11
Aplysia Ganglia Preparation for Electrophysiological and Molecular Analyses of Single Neurons
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Cited by 9 •
2014
Marine snail Aplysia californica has been widely used as a neurobiology model for the studies on cellular and molecular basis of behavior. Here a methodology is described for exploring the nervous system of Aplysia for the electrophysiological and molecular analyses of single neurons of identified neural circuitry.
FAQs
Jitter measures the timing variation between electrical signals from neighboring muscle fibers. This variability provides information about how consistently signals pass across the neuromuscular junction, where nerve activity activates muscle fibers. Increased or abnormal jitter can therefore reveal transmission problems that may be difficult to detect with less sensitive electrophysiological testing.
Timing measurements show when individual muscle fibers generate their electrical responses, while waveform features describe the shape of those responses. Examining both characteristics helps characterize the recorded single-fiber action potentials rather than relying only on signal timing. Together with variability measurements, they provide a more detailed view of neuromuscular function.
Single Fiber Electrophysiology focuses on electrical activity from individual muscle fibers and can detect abnormalities that conventional electromyography or nerve-conduction studies may not show. Its particular value comes from measuring single-fiber timing, waveform characteristics, and jitter, allowing more sensitive evaluation of transmission across the neuromuscular junction.
Recordings can be obtained while the patient voluntarily activates the muscle or while a nerve is stimulated. These approaches provide different ways to produce and observe single-fiber action potentials. The selected condition allows the examiner to assess signal timing, waveform features, and variability under controlled muscle activation or nerve-driven activation.
Clinicians may use the method when they need sensitive information about neuromuscular-junction transmission in a patient being assessed for myasthenia gravis. Measurements such as jitter can reveal irregular signal transmission between neighboring fibers. The findings contribute to the electrophysiological evaluation of the disorder rather than serving as a general measurement of muscle activity alone.
Beyond myasthenia gravis, the technique can support assessment of conditions affecting peripheral nerves, motor neurons, or muscle. Its measurements help determine whether abnormalities are present in neuromuscular function and transmission. This broader clinical role makes the method relevant when routine electrophysiological studies do not fully explain suspected neuromuscular dysfunction.