Syringe Emulsification
Syringe Emulsification - Related Videos
11:03
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
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Cited by 1 •
2021
Water-in-oil droplet assays are useful for analytical chemistry, enzyme evolution, and single cell analysis, but typically require microfluidics to form the droplets. Here, we describe particle templated emulsification, a microfluidic-free approach to perform droplet assays.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
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Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
05:49
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Cited by 2 •
2021
Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.
09:08
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Cited by 3 •
2018
Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.
FAQs
Mechanical shear is generated when the formulation moves repeatedly between connected syringes. This force breaks one immiscible liquid into smaller droplets dispersed within the other. The resulting droplet distribution makes the preparation more uniform than a simple, unmixed combination. In medicine, this uniformity supports more consistent handling and helps active ingredients be delivered predictably.
Emulsifying agents help stabilize the mixture after the liquids have been dispersed. Without adequate stabilization, the uniformity created during mixing may be less reliable during handling. Their use is therefore relevant when developing formulations intended to remain sufficiently consistent for medical or pharmaceutical purposes, including topical, oral, or other dosage forms.
The transfer fitting creates the connected pathway through which the two liquids move during processing. Securing this connection supports repeated back-and-forth transfer, allowing mechanical shear to act on the immiscible phases. This component is especially important in a small-scale workflow because the quality of the transfer directly affects whether the materials can be combined uniformly.
A typical workflow places the two immiscible liquids in connected syringes, uses a secure transfer fitting, and repeatedly moves the contents between them. Each transfer applies mechanical shear that disperses one liquid into the other. The process continues until the preparation reaches the desired degree of uniformity, with an emulsifying agent used when stabilization is needed.
Syringe Emulsification is useful for extemporaneous preparation and formulation development when only a small amount of material must be combined. The approach can support work on topical, oral, or other dosage forms. Its practical value comes from combining small-scale handling with improved homogeneity, which can help investigators assess or prepare formulations containing active ingredients.
A consistent emulsion can improve homogeneity, meaning the ingredients are distributed more evenly throughout the preparation. It can also make the formulation easier to handle and support more predictable delivery of active ingredients. These outcomes matter because nonuniform mixtures may provide less reliable preparation characteristics, whereas a stable, consistent mixture better supports intended medical use.