Chemokine Expression
Chemokine Expression - Related Videos
10:02
An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
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2019
To test the effect of a chemokine on macrophage recruitment in vivo, the whole mount in situ hybridization was used to detect the ectopic expression of the chemokine, and immunostaining was used to label macrophages. Live imaging was used for real-time observation of macrophage migration.
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
06:48
Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses
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Cited by 1 •
2020
Here we describe protocols to perform live imaging and quantitative analysis of chemoattractant receptor dynamics in zebrafish...
06:56
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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Cited by 22 •
2018
A flow cytometry-based cellular binding assay is described that is primarily used as a screening tool to identify compounds that inhibit the binding of a fluorescently labeled CXC chemokine ligand 12 (CXCL12) to the CXC chemokine receptor 4 (CXCR4).
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
FAQs
Infection, injury, and other cellular signals can shift transcriptional pathways so that chemokine production increases or decreases. This regulation allows tissues to adjust communication and recruitment of responsive cells rather than maintaining a fixed signal level. In neuroscience, examining these changes helps connect a local disturbance with inflammatory responses in nervous-system tissue.
Chemokine signaling depends on both receptor recognition and spatial organization. Secreted chemokines bind specific G protein-coupled receptors, while concentration gradients provide directional information to responsive cells. Consequently, chemokine release can signal that a disturbance exists while also influencing where responsive cells move during inflammation or nervous-system injury.
Microglia, astrocytes, and neurons can contribute chemokine signals within nervous tissue, while infiltrating immune cells provide an additional source. Considering these populations separately matters because expression patterns can reflect resident-cell activity, incoming immune responses, or both. This distinction helps researchers interpret neuroinflammation, immune-cell recruitment, and injury responses more precisely.
Specific receptor binding determines which responsive cells can detect a chemokine signal. Because secreted chemokines act through particular G protein-coupled receptors, this specificity helps connect a producing cell or tissue region with the cells able to respond. Combined with concentration gradients, receptor-specific signaling influences recruitment patterns during neuroinflammation and nervous-system injury.
Researchers can compare chemokine patterns across microglia, astrocytes, neurons, and infiltrating immune cells while relating them to conditions such as infection, injury, or other cellular signals. They can then assess whether expression rises or falls and whether the pattern aligns with neuroinflammation, immune-cell recruitment, synaptic function, or responses to nervous-system injury.
Expression measurements can show how signaling changes accompany neuroinflammation, immune-cell recruitment, synaptic function, or responses to nervous-system injury. Interpreting these patterns does more than record the presence of chemokines; it can help connect particular cellular sources and regulatory changes with disease-related processes. Such evidence may also support evaluation of potential therapeutic targets.
Patterns of regulated chemokine production can provide mechanistic context for evaluating potential therapeutic targets. Researchers can relate increases or decreases in expression to the activity of microglia, astrocytes, neurons, or infiltrating immune cells, as well as to neuroinflammation, recruitment, synaptic function, and injury responses. This connects target evaluation with measurable cellular signaling changes in nervous-system disease.