Endothelial Permeability
Endothelial Permeability - Related Videos
07:03
An in vivo Assay to Test Blood Vessel Permeability
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Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
02:53
Blood-Brain Barrier Mimic Permeability Assay: An In Vitro Assay to Assess the Permeability of BBB Mimic to Tracer Molecules
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2023
This video describes a permeability assay to evaluate the permeability of in vitro blood-brain barrier using sodium fluorescein molecules.
06:26
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
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Cited by 6 •
2020
The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial traversal.
06:37
An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium
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Cited by 11 •
2012
We describe a live whole animal quantitative measurement for permeability of the embryonic zebrafish brain. The technique analyzes the ability to retain cerebrospinal fluid and molecules of different molecular weights within the neural tube lumen and quantifies their movement out of the ventricles. This method is useful for determining differences in epithelial permeability and maturation during development and disease.
12:48
Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery
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Cited by 3 •
2015
The modified Landis technique enables paired measurement of the hydraulic conductivity of individual microvessels in the mesentery of normal and genetically modified rats under control and test conditions using microperfusion techniques. It provides a convenient method to evaluate mechanisms that regulate microvessel permeability and transvascular exchange under physiological conditions.
FAQs
Barrier selectivity emerges from the combined behavior of tight junctions and adherens junctions between endothelial cells. These junctions regulate whether substances pass through the spaces between cells, while their properties help maintain vascular homeostasis. Changes in junctional organization can therefore alter the movement of fluids, ions, molecules, and cells across the endothelial layer.
Vesicular transport provides a route for substances to move across endothelial cells rather than between them. The extracellular matrix also contributes by interacting with the endothelial layer and influencing barrier properties. Considering both mechanisms is important because permeability reflects coordinated control of junctional pathways, vesicular movement, and endothelial interactions with surrounding tissue.
Specialized brain endothelial cells form the blood-brain barrier, where permeability is tightly selective to protect neural tissue from uncontrolled exchange with the circulation. Studying this selectivity helps clarify how vascular barriers respond in the nervous system and supports investigation of approaches intended to deliver therapeutic substances to the brain.
Altered permeability can change the passage of fluids, ions, molecules, and cells across the vascular lining. In neural tissue, such changes are relevant to neuroinflammation and edema because the blood-brain barrier normally limits entry into the brain. Measuring permeability therefore helps connect vascular barrier disruption with disease-related changes in the nervous system.
Permeability measurements indicate whether the endothelial barrier has become more or less restrictive to exchange. In neuroscience, these measurements can help characterize vascular changes associated with neuroinflammation, edema, stroke, and tumor biology. They also provide a way to evaluate barrier behavior when developing strategies for targeted delivery to neural tissue.
Studies of endothelial permeability identify how barrier properties control access to neural tissue. This information can guide strategies that seek to deliver drugs to the brain while accounting for the selective behavior of specialized brain endothelial cells. The same measurements can also help assess how disease-associated changes in the barrier may affect delivery opportunities.