Fungal Beta-glucan
Fungal Beta-glucan - Related Videos
10:02
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
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Cited by 1 •
2023
The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.
02:25
An Assay for Analyzing Test β-Glucan as an Immunotherapy Strategy against Brain Cancer Cells
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2025
This video demonstrates an in vitro assay utilizing β-glucans to activate microglial cells for the production of superoxide radicals, a reactive oxygen species. In brain cancer cells, the generated reactive oxidants actively suppress tumor cell proliferation and induce cell death.
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
03:51
Establishing Bacterial–Fungal Cross-Kingdom Biofilms for Studying Oral Pathogenesis
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2026
Source: Schlafer, S, and Frost Kristensen, M. Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy. J. Vis. Exp. (2020)This video demonstrates the cultivation of Streptococcus mutans and Candida albicans cross-kingdom biofilms under oral-like conditions to model bacterial–fungal interactions, enabling the study of microbial synergy, glucan-mediated adhesion, and acidogenic virulence in oral pathogenesis.
07:31
Microbial and Fungal Diversity - Student Protocol
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2019
Examining Microbial Colony Structures ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them. Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate. As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1 Then, repeat the observations for each of the other two bacterial species.
FAQs
Fungal beta-glucan can be recognized by innate immune pattern-recognition receptors, particularly Dectin-1. Receptor engagement initiates signaling that changes cytokine production and immune-cell behavior. In neuroscience experiments, this provides a mechanistic link between an exposure event outside the nervous system and downstream questions about microglial responses or neuroinflammation.
These variables can alter the biological activity observed after exposure. Structural differences may affect receptor detection, while dose can influence the magnitude of immune signaling. The route determines how the carbohydrate is encountered by the organism or experimental system. Controlling these factors is therefore essential when comparing cytokine responses, immune-cell behavior, or nervous-system effects.
Dectin-1 functions as an innate immune pattern-recognition receptor that detects fungal beta-glucan. Its activation helps connect carbohydrate exposure with intracellular signaling, cytokine production, and changes in immune-cell behavior. Studying this receptor gives investigators a focused way to examine how recognition of a fungal cell-wall component may initiate signals relevant to neuroinflammation and immune-to-nervous-system communication.
A useful design begins by specifying the fungal beta-glucan exposure, including its molecular structure, dose, and route. Investigators can then examine receptor-linked immune signaling, cytokine production, and immune-cell behavior. In neuroscience-focused work, the design can extend to questions about microglia, neuroinflammation, or brain function, while keeping exposure conditions consistent for meaningful comparisons.
It serves as a model stimulus for investigating how peripheral immune activity interacts with the nervous system. Researchers can use the resulting immune signaling to study relationships among cytokine production, microglial behavior, neuroinflammation, and brain function. This approach does not treat the carbohydrate only as an immune stimulus; it also probes communication between peripheral immune processes and neural systems.
These studies can reveal how an immune-recognition event influences cytokine production and immune-cell behavior, and whether those signals are associated with microglial or neuroinflammatory changes. Interpretation must account for the carbohydrate's molecular structure, dose, and exposure route because different conditions may produce different outcomes. Such comparisons help clarify immune-to-nervous-system communication.