Nanoliter Injection
Nanoliter Injection - Related Videos
09:32
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
0 Views •
Cited by 66 •
2013
Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.
05:06
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
0 Views •
Cited by 10 •
2021
This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
08:28
Zebrafish Brain Ventricle Injection
0 Views •
Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
09:19
Intraperitoneal Injection into Adult Zebrafish
0 Views •
Cited by 238 •
2010
We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.
04:51
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
0 Views •
2025
This video demonstrates a method to generate a rat model of neonatal intraventricular hemorrhage. In this procedure, an anesthetized rat pup is injected with hemoglobin into the lateral ventricle of the brain. The hemoglobin causes oxidative stress and releases heme, simulating intraventricular hemorrhage. The resulting damage to brain tissues, driven by reactive oxygen species and inflammatory cytokines, leads to ventricular enlargement, a common consequence of intraventricular hemorrhage.
FAQs
The delivered volume is regulated by applying controlled pressure or mechanical displacement to the liquid within the fine glass micropipette. Calibration links the applied movement or pressure to the amount expelled, allowing researchers to introduce a defined nanoliter-scale volume rather than relying on uncontrolled release. This control is essential when dose and local exposure must remain consistent.
Positioning determines whether the reagent reaches the intended neuron or brain region and how much surrounding tissue is exposed. A fine glass micropipette can be placed at a selected cellular or anatomical target before solution delivery begins. Accurate placement therefore supports localized manipulation while helping limit effects outside the experimental site.
Small, calibrated deliveries reduce the amount of solution introduced around the target compared with a less localized application. When the micropipette is accurately positioned and expulsion remains controlled, exposure can be concentrated near the selected neuron or brain region. This localized delivery helps researchers distinguish target-specific effects from broader changes in surrounding neural tissue.
A typical workflow begins by loading the selected solution into a fine glass micropipette, positioning the pipette at the intended neuron or brain region, and calibrating the delivery system. The researcher then applies controlled pressure or mechanical displacement to expel the planned volume. The procedure is designed to coordinate placement and delivery so the target receives a localized dose.
The core setup includes a fine glass micropipette and a mechanism that produces controlled pressure or mechanical displacement. The pipette must be positioned at the chosen cellular or tissue target, while the delivery system must release a calibrated volume. Together, these components establish the spatial and volumetric control required for microscale neural experiments.
In neuroscience, the technique can deliver tracers, pharmacological agents, genetic materials, or other reagents to selected neurons or brain regions. These localized interventions support studies of neural circuits, cellular signaling, and brain function. Because delivery is spatially restricted, resulting changes can be related more directly to the targeted cells or tissue than to widespread exposure.