Gabaergic Neuron Transplantation
Gabaergic Neuron Transplantation - Related Videos
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Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
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Cited by 2 •
2022
Transplantation of human pluripotent stem cell-derived GABAergic neurons generated by neuronal programming could be a potential treatment approach for neurodevelopmental disorders. This protocol describes the generation and transplantation of human stem cell-derived GABAergic neuronal precursors into the brains of neonatal mice, allowing long-term investigation of grafted neurons and evaluation of their therapeutic potential.
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Transplantation of Stem Cell-Derived Neuronal Progenitors into Human Cortical Slices
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2025
Source: Palma-Tortosa, S., et.al. Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation. J. Vis. Exp. (2022)This video demonstrates the transplantation of stem cell-derived neuronal progenitor cells into human cortical tissue slices. The procedure involves isolating and resuspending progenitor cells in a basement membrane matrix, followed by their injection into a cortical slice placed on a transwell assembly. After incubation to allow...
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
09:21
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
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Cited by 35 •
2015
GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.
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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
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Cited by 9 •
2019
This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.
FAQs
The main functional effect comes from adding inhibitory signaling to a circuit whose activity is excessive or poorly balanced. After transplantation, GABA-producing cells can mature, extend processes, and connect with host neurons. Those connections allow the graft to influence existing network activity rather than acting only as a separate cell population. This distinction is important when evaluating circuit repair.
Cell maturation determines whether implanted precursors can contribute to circuit function. Immature cells must develop into inhibitory neurons and establish processes and synaptic connections with host neurons before their influence can be assessed. Consequently, early observations may not represent the eventual effect of the graft, making maturation and connectivity central variables in neuroscience studies.
Outcome depends on several linked variables rather than on cell delivery alone. The source of the cells, their survival after transplantation, their ability to integrate, and the accuracy of placement can each influence functional results. A graft that survives but fails to connect appropriately may not restore balance, whereas successful integration could alter abnormal network activity.
Precise placement determines which damaged or hyperexcitable circuit receives the new inhibitory cells. It can influence whether the graft reaches relevant host neurons, extends processes through the intended region, and forms useful synaptic connections. For that reason, anatomical location is not merely a delivery detail; it is a major experimental variable when interpreting changes in network activity.
A basic experimental workflow includes choosing an appropriate cell source, introducing inhibitory neurons or their precursors into the selected brain region, and then evaluating what happens over time. Investigators can examine cell survival, maturation, process extension, synaptic connection with host neurons, and changes in network activity. These measurements link transplantation to circuit-level function.
Epilepsy research provides a major application because seizures are associated with excessive excitation in neural circuits. In this context, investigators ask whether added inhibitory signaling can reduce abnormal network activity and whether the graft can contribute to longer-term circuit repair. The approach remains experimental, so efficacy and safety require continued study.
Researchers interpret success through more than the presence of transplanted cells. Relevant outcomes include whether cells survive, mature, extend processes, connect functionally with host neurons, and influence abnormal activity. Long-term safety is also essential, because an apparent short-term change does not by itself demonstrate stable, appropriate circuit integration or therapeutic value.