Murine Models
Murine Models - Related Videos
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
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2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
03:47
A Murine Model of Carotid Aneurysm Formation
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2025
The present protocol describes a standardized surgical method for the induction of carotid aneurysms in a murine model through a single surgical operation.
09:07
Murine Model of Epicutaneously-Induced Immunomodulation
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2025
The study demonstrates the role of the skin in modulating immune responses in mice through epicutaneous (EC) immunization. EC immunization with a protein antigen can suppress immune responses in contact hypersensitivity or reverse skin-induced suppression when combined with pathogen-associated molecular patterns. EC immunization shows promise as an effective, needle-free approach to immunotherapy.
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
03:06
A Method to Induce Ocular Surface Inflammation in a Murine Model
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2025
This video demonstrates a method to induce ocular surface inflammation and meibomian gland dysfunction in a murine model. Stimulation of the mouse's immune system using an immunogen and later restimulation with the same immunogen promotes ocular surface inflammation, which causes plugging of meibomian gland secretion through neutrophil-produced aggNETs and leads to dry eye disease.
FAQs
A murine model can connect pharmacokinetics, the study of absorption, distribution, metabolism, and excretion, with pharmacodynamics, the study of drug effects. Measuring both after defined dosing helps investigators relate compound exposure to target engagement, dose-response relationships, and observed biological outcomes. This combined view supports interpretation of whether a response reflects sufficient exposure, target activity, or emerging toxicity.
Genetically modified murine models can clarify disease pathways or test the contribution of a proposed drug target. By examining pharmacological responses in a model with a defined genetic alteration, investigators can assess whether changes in that pathway alter efficacy, target engagement, or other measured outcomes. These comparisons provide mechanistic context that standard disease models may not supply.
Species differences can limit how directly findings from mice translate to human patients. Differences affecting drug response may influence the interpretation of efficacy, pharmacokinetics, pharmacodynamics, or toxicity results. Consequently, a favorable outcome in a murine model supports preclinical evaluation but does not by itself establish that the same compound, dose-response relationship, or safety profile will occur in people.
Investigators administer a compound at defined doses and then measure relevant pharmacokinetic and pharmacodynamic outcomes. These measurements may include absorption, distribution, metabolism, excretion, target engagement, dose-response relationships, and toxicity. The resulting data are examined together to evaluate the compound's biological activity and safety, creating a preclinical evidence base for decisions about further study.
Murine models are used before clinical studies to evaluate therapeutic efficacy, safety, mechanisms of action, and biomarkers. They can also help determine whether a compound reaches and influences its intended target and whether responses vary with dose. This combination of efficacy and safety information helps characterize a candidate treatment before investigators consider translation to human research.
Studies in mice can provide evidence about whether a compound produces a measurable therapeutic response, engages a biological target, follows a definable dose-response relationship, or produces toxicity. They can also identify pharmacokinetic patterns and potential biomarkers associated with treatment. Interpreted together, these outcomes help distinguish drug activity from safety concerns and clarify the compound's mechanism of action.