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August 14, 2012
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We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.
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Wong, C., Vosburgh, E., Levine, A. J., Cong, L., Xu, E. Y. Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy. J. Vis. Exp. (66), e4218, doi:10.3791/4218 (2012).
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