Name: lab waste management and safety
Uploaded: 2023-04-30T06:59:24.000Z
Duration: 4 min 28 s
Channel: JoVE

K12 Standards

north-carolina-k-12-science-standard-course-of-study

North Carolina K-12 Science Standard Course of Study

9-12

Earth and Environmental Science

Earth and Human Activity

ESS.EES.5.5

Proper Waste Disposal

1. Gestão de Resíduos
<ol><li>Os usuários devem fazer um esforço para manter o desperdício ao mínimo. A melhor maneira de fazê-lo é reduzindo a escala de operação, o que minimiza a quantidade de resíduos gerados. Sempre que possível, os produtos químicos utilizados devem ser substituídos por produtos químicos menos perigosos.</li>
	<li>As quantidades químicas devem ser mantidas ao mínimo. Armazene apenas o que será usado no curto prazo.</li>
	<li>Além de prevenir ou minimizar a geração de resíduos, os produtos químicos devem ser reciclados ou recuperados para reutilização.</li>
	<li>Quando o lixo é gerado, deve ser descartado corretamente. O descarte de pias pode nem sempre ser apropriado e pode acabar contaminando a água potável. Devem ser considerados métodos alternativos de descarte, incluindo incineração, tratamento e destinação de terrenos. O escritório de EHS do instituto deve ser consultado para determinar o método adequado de descarte para diferentes tipos de resíduos.</li>
</ol>2. Coleta e Armazenamento de Resíduos
<ol><li>Ao gerar ou gerenciar quaisquer resíduos químicos, devem ser usados equipamentos de proteção individual (EPI) adequados e os controles de engenharia devem ser implementados conforme necessário.</li>
	<li>Coletar e armazenar resíduos químicos no ponto de geração ou próximo em uma área designada de acumulação de satélites. Esta área de acumulação deve ser bem marcada para fácil identificação.</li>
	<li>Os resíduos químicos devem ser armazenados em recipientes compatíveis com tampas fechadas e adequadamente instaladas.</li>
	<li>Os recipientes de resíduos devem ser rotulados mencionando composições químicas, a data de início do acúmulo e avisos de perigo conforme apropriado. O escritório de EHS do instituto normalmente fornece esses rótulos necessários.</li>
	<li>Os tipos de resíduos incompatíveis não devem ser misturados e devem ser mantidos separados para evitar qualquer reação, geração de calor e/ou evolução do gás.</li>
	<li>Os recipientes de resíduos devem ser armazenados em recipientes secundários em área ventilada, fria e seca.</li>
	<li>Na área central de acumulação, os recipientes de resíduos devem ser aterrados para evitar riscos de incêndio e explosão.</li>
	<li>Pesquisadores de laboratório treinados que estejam mais familiarizados com os resíduos gerados devem trabalhar com a EHS para garantir a gestão adequada dos resíduos.</li>
</ol>3. Descarte de sharps - Seringas e Agulhas
<ol><li>Agulhas, seringas e lâminas de barbear quimicamente contaminadas devem ser descartadas dentro de um recipiente de afiadas adequados.</li>
	<li>Seringas ou agulhas nunca devem ser descartadas em uma lixeira de laboratório ou em um recipiente de resíduos em geral.</li>
</ol>4. Reciclagem de vidro
<ol><li>A reciclagem de vidro é amigável ao meio ambiente, pois reduz a poluição causada pelos resíduos que acabam em aterros sanitários. Todo laboratório deve ter uma lixeira separada dedicada ao vidro.</li>
	<li>Garrafas de vidro vazias limpas e vidros quebrados podem ser reciclados. Para limpar uma garrafa de vidro vazia, ela deve ser "enxaguada triplamente" com água ou outro solvente adequado e seco a ar antes do descarte.</li>
	<li>Vidros de laboratório quimicamente contaminados, como tubos de amostra, conta-gotas e lã de vidro devem ser descartados como resíduos controlados.</li>
</ol>

Neste documento, é fornecida uma diretriz básica para o descarte de resíduos laboratoriais. Os usuários devem trabalhar e cumprir o escritório de EHS de seu instituto para determinar o método adequado para o descarte de resíduos que satisfaça diversas regulamentações e normas. O usuário do laboratório deve estar ciente do que o material resíduo está sendo gerado e os perigos presentes devem ser cuidadosamente avaliados para determinar o descarte adequado de resíduos, o que pode colocar a saúde pública ou o meio ambiente ...

descarte adequado de resíduos

Robert M. Rioux e Taslima A. Zaman, Universidade Estadual da Pensilvânia, Pensilvânia, PA

Os usuários são responsáveis pelo descarte adequado dos resíduos gerados durante seu trabalho. O descarte inadequado de resíduos pode colocar em risco severamente a saúde pública e/ou o meio ambiente. O manuseio de resíduos perigosos deve ser regulado a partir do momento da geração até sua disposição em sua instalação de destino final offsite. Um sistema de gestão de resíduos deve ser elaborado antes do início dos trabalhos em qualquer atividade laboratorial. Os usuários devem cumprir as regras e regulamentos do escritório de Saúde e Segurança Ambiental (EHS) de seu instituto, que desenvolve e implementa sistemas adequados de gestão de resíduos que satisfaçam diversas normas e normas, como as impostas pela Administração de Segurança e Saúde Ocupacional (OSHA).

Descarte adequado de resíduos

Robert M. Rioux and Taslima A. Zaman, Pennsylvania State University, Pennsylvania, PA

Users are responsible for the proper disposal of the waste ...

Resíduos perigosos, sejam químicos, médicos ou radioativos, são gerados em muitos laboratórios e requerem descarte regulamentado para garantir a segurança da saúde pública e do meio ambiente. A regulamentação do manuseio de resíduos perigosos deve ser aplicada desde o momento da geração até seu descarte em uma instalação de destino final externa. Antes de iniciar qualquer atividade laboratorial, deve ser elaborado um sistema de gerenciamento de resíduos. Isso geralmente é estabelecido pelo escritório de Saúde e Segurança Ambiental de um instituto, ou EH&S, que aplica as diretrizes impostas pela Administração de Segurança e Saúde Ocupacional, ou OSHA. Este vídeo ilustrará os princípios e procedimentos laboratoriais típicos de descarte adequado de resíduos. A gestão eficiente de resíduos é um aspecto importante do descarte adequado de resíduos. Isso pode ser feito usando a quantidade mínima de produtos químicos possível, reutilizando materiais excedentes e reciclando resíduos. Os resíduos gerados devem ser rotulados, segregados de acordo com a compatibilidade química e armazenados em capela ou outra área bem ventilada. Outros resíduos de laboratório, como perfurocortantes e vidros, devem ser descartados com cuidado em recipientes apropriados. Agora que discutimos os princípios do descarte adequado de resíduos, vejamos um procedimento real. Se aplicável, reduza ao mínimo os resíduos químicos, reduzindo a escala de operação. Além disso, substitua os produtos químicos por reagentes menos perigosos sempre que possível. Além de minimizar a escala de operação, armazene apenas as quantidades químicas que serão usadas no curto prazo. Você também pode reduzir o desperdício químico reciclando solventes como a acetona, usando uma destilação. Use equipamento de proteção individual adequado, incluindo jaleco, óculos e luvas, bem como calças compridas e sapatos fechados, sempre que manusear qualquer resíduo químico. Colete resíduos químicos em recipientes adequados, como garrafões de plástico ou garrafas de vidro, e armazene perto do ponto de geração em uma área designada de acumulação de satélites. Afixe etiquetas nos recipientes de lixo assim que os produtos químicos forem adicionados. Escreva nos rótulos os nomes completos dos produtos químicos e suas composições aproximadas. Além disso, use recipientes separados para resíduos halogenados, não halogenados e aquosos para evitar a formação potencial de calor ou gás. Quando os recipientes estiverem cheios até a capacidade máxima, mova-os cuidadosamente para uma área de acumulação central designada, de onde serão removidos para descarte. Descarte agulhas, seringas e lâminas de barbear quimicamente contaminadas, conhecidas coletivamente como perfurocortantes, dentro de um recipiente de lixo perfurocortante. Para vidro quebrado, pipetas ou tubos de ensaio usados, use um recipiente de lixo de vidro especializado. Se desejar, as garrafas vazias podem ser reutilizadas após o triplo aumento com acetona, água e novamente acetona. Você acabou de assistir à introdução de JoVE ao descarte adequado de resíduos. Agora você deve entender o gerenciamento de resíduos, como coletar resíduos químicos e perfurocortantes e como armazená-los para descarte. Obrigado por assistir!

Education

JoVE Science Education

Basic Biology

Biologia Básica

Lab Safety

Segurança em Laboratório

lab waste management and safety

Lab waste management and safety are essential parts of any laboratory activity. Waste generated during work must be handled correctly from the moment it is created until it reaches its final offsite disposal facility. Poor waste disposal can seriously harm public health and the environment.

A waste management system should be planned before any lab work begins. This system guides how waste is collected, stored, and prepared for removal. For hazardous waste, special rules apply because its handling must be regulated throughout the entire process.

Students and lab users must also follow their institution’s Environmental Health and Safety, or EHS, office. The EHS office develops and carries out waste management systems that meet different rules and standards. These include requirements set by the Occupational Safety and Health Administration, or OSHA.

Lab Waste Management and Safety

proper personal protective equipment

Choosing the Right Lab PPE

Choosing the right lab PPE is a key part of working safely in a laboratory. PPE, or personal protective equipment, helps reduce risk when hazards are present. Laboratory hazards can be many and varied. The right choice of PPE helps make the lab a safe place to work. Students should match their protective gear to the tasks and conditions in the lab. This topic focuses on selecting proper protection for the situation at hand. Wearing the correct PPE supports safer lab work and helps limit...

emergency eyewash and shower stations

Laboratory Chemical Spill Response Stations

Laboratory chemical spill response stations help protect people who are exposed to injurious or corrosive substances. In workplaces and school labs, OSHA requires emergency eyewash and shower stations to be easy to reach when these hazards are present. These stations are used after an accident that spills a harmful chemical on the body or splashes it into the eyes. The eyewash station helps rinse the eyes, while the shower station is used for chemicals on the skin or clothing. They are meant...

electrical safety

Preventing Lab Electric Shock and Fires

Electrical hazards are a common lab safety concern. Most laboratory equipment uses electricity to work, so students and lab staff must stay alert around cords, plugs, and powered devices. Improper use of electrical equipment can cause electric shock. In serious cases, this can lead to injury or death. Electrical sparks are also dangerous in the lab. They can start a fire or trigger an explosion. This risk matters because many flammable chemicals may be stored near powered instruments. Knowing...

working with centrifuges

Centrifuge Safety and Sample Separation

Centrifuges are used to separate heterogeneous mixtures by spinning particles in a fluid at high speed. The motion creates a force away from the axis of rotation. Heavier particles move faster toward the bottom of the sample holder, and the rate depends on particle size and density. This process is called centrifugation, and it speeds up sedimentation, which is the settling of particles out of a liquid. Gravity can separate some mixtures on its own, but that can take a long time.

working with hot and cold sources

Safe Use of Lab Heat and Cold Sources

Safe use of lab heat and cold sources is an important part of laboratory work. Extreme temperatures are common in many lab tasks. They can involve both high heat and very low temperatures. Bunsen burners and hot plates are widely used in research labs and industry. A Bunsen burner is a gas burner that gives a direct flame. A hot plate is an electric device that warms containers without an open flame. Because these tools reach high temperatures, users need to follow safe handling procedures. <br...

guidelines in case of a laboratory emergency

guidelines-in-case-of-a-laboratory-emergency

Lab Spill and Accident Response Basics

Lab spill and accident response basics help students stay safe during common laboratory emergencies. The most common lab emergencies include chemical spills, fire or explosion, electric shock, and personnel injuries. Many accidents happen because of poor planning or lack of attention, so prevention is always the first step. Good lab safety starts before an emergency happens. Students should wear proper personal protective equipment, or PPE, in the laboratory. Regular lab inspection and...

chemical storage: categories, hazards and compatibilities

chemical-storage-categories-hazards-and-compatibilities

Safe Chemical Labels and Storage Basics

Safe chemical storage and labeling help prevent dangerous reactions in school and research labs. Chemicals can vary widely in their properties and reactivity, so they must be managed, stored, and labeled correctly as part of an Environmental, Health and Safety (EHS) program. If chemicals are not handled properly, they can create harmful or even destructive results. These risks include toxic fume production, fire, explosion, human fatality, property damage, and environmental hazards. Proper...

safe handling of mineral acids

Mineral Acids in the Lab

Mineral acids are common chemicals in research laboratories, and they must be handled with care. These acids, also called inorganic acids, are water-soluble acids formed from inorganic minerals by chemical reaction. Their corrosive nature makes them a major safety risk in the lab. Examples of mineral acids include boric acid, chromic acid, hydrochloric acid, hydrofluoric acid, nitric acid, perchloric acid, phosphoric acid, and sulfuric acid. These acids are important reagents in research, and...

handling chemical spills

Chemical Spill Safety in the Lab

Chemical spill safety in the lab is essential whenever hazardous chemicals are used. Spills can happen at any time during laboratory work. Even a small spill can become dangerous if people are not prepared to respond quickly and correctly. Preparing for a chemical spill helps protect public health and the environment. Lab teams need to know how to respond to accidental releases of hazardous chemicals before an emergency happens. Safe cleanup also depends on proper disposal of the spilled...

proper use of autoclaves

Autoclaving Lab Materials Safely

Autoclaving lab materials safely is an important part of decontamination in the laboratory. This method is commonly used for items that have contacted biological samples, especially those that may contain microorganisms. An autoclave cleans materials with pressurized steam at a high temperature. The steam and heat work together to kill microorganisms on the loaded items. For this reason, effective autoclaving depends on good steam flow and strong heat transfer. Packaging matters because it...

fume hoods and laminar flow cabinets

Airflow Controls for Safer Lab Workspaces

Fume hoods and laminar flow cabinets are airflow controls used in laboratories. They both rely on a constant stream of air to protect the lab environment and the people in it. These systems work in similar ways, but they serve different purposes. A fume hood is a ventilation system that helps reduce exposure to hazardous vapors, fumes, and particles. Air is drawn into the hood opening, which helps keep harmful substances inside the workspace. The air is then pulled out through the exhaust. A...

handling air- and water-sensitive chemicals using a schlenk line

handling-air-and-water-sensitive-chemicals-using-a-schlenk-line

Schlenk Line Setup for Inert Atmospheres

A Schlenk line helps chemists work with air- and water-sensitive chemicals. It provides an air-free way to handle reagents that can react with oxygen or moisture. This makes it useful in a chemical laboratory when careful control of the atmosphere is needed. The glass apparatus is commonly used because it can create an inert gas environment or a vacuum. Inert gases such as nitrogen and argon can be used to protect the reaction. The same setup can also expose glassware to vacuum when needed. A...

proper operation of vacuum based equipment

proper-operation-of-vacuum-based-equipment

Vacuum Pumps for Laboratory Procedures

Vacuum pumps are used in many laboratory procedures that need low pressure. They help create vacuum conditions for experiments and equipment that must run below normal atmospheric pressure. In the lab, vacuum pumps are the most common way to achieve this low-pressure environment. They can also support rapid changes in the atmosphere inside a reactor or flask. This is done by evacuating the vessel and then backfilling it with another gas.

operating the glovebox

Glovebox Atmosphere and Sample Transfer

A glovebox is an enclosed workspace that keeps an inert atmosphere inside. Scientists use built-in gloves to handle chemicals or equipment without exposing the inside of the box to outside air. The box uses positive pressure to help make up for small passive leaks. Objects can be moved in and out through an antechamber, which works like an airlock between the glovebox and the outside atmosphere. Some gloveboxes have more than one pair of gloves. The number of glove ports depends on the size...

operation of high-pressure reactor vessels

operation-of-high-pressure-reactor-vessels

Safe Use of Pressure Vessels in Chemistry

High-pressure reactor vessels are used in synthetic chemistry to handle gases safely during reactions. They help chemists carry out gas-based processes such as hydrogenation, oxidation, and amination. These reactions often use hydrogen, oxygen, or ammonia, which are important reagents in the lab. These gases usually dissolve poorly in standard reaction mixtures. Because of that, high pressure is needed to make the reaction proceed at a useful rate. The vessel creates a sealed space that keeps...

decontamination for laboratory biosafety

Lab Cleaning Methods for Biosafety

Laboratory cleaning methods are a key part of biosafety because microbial contamination can build up and spread disease. Decontamination is used to reduce that risk on lab surfaces and equipment. The level of cleaning depends on how much contamination is present and what kind of item needs treatment. Decontamination can be grouped into disinfection and sterilization. Disinfection removes pathogenic microorganisms, but it does not remove bacterial spores on lab surfaces or equipment.

proper waste disposal

Lab waste management and safety are essential parts of any laboratory activity. Waste generated during work must be handled correctly from the moment it is created until it reaches its final offsite disposal facility. Poor waste disposal can seriously harm public health and the environment. A waste management system should be planned before any lab work begins. This system guides how waste is collected, stored, and prepared for removal. For hazardous waste, special rules apply because its...

lab safety

Esta coleção fornece diretrizes de segurança a serem seguidas ao trabalhar com materiais e equipamentos perigosos. Abrange temas universais como EPI, segurança elétrica e orientações gerais de emergência, além de alguns procedimentos específicos de segurança para laboratórios de química e biologia.

Learn why lab waste must be managed from creation to final disposal, and how EHS and OSHA rules help protect health and the environment.

Lab Waste Management and Safety | JoVE K12

Louisiana Student Standards for Science

High School

Earth Science

Human Sustainability

HS-ESS3-4

Virginia  Standards of Learning

HIgh School

ES.6

ES.6.a

Texas Essential Knowledge and Skills

Earth Systems Science

112.49.c.13.

112.49.c.13.A.

Specialised Topic in Science

112.51.c.1.

112.51.c.1.C.

Florida Department of Education

Life Science

SC.912.L.17

SC.912.L.17.14

Biology

BIO.1

BIO.1.C

Chemistry and Physics

IPC.1

IPC.1.C

new-york-state-science-learning-standards

New York State Science Learning Standards

engineering-technology-and-applications-of-science

Engineering, Technology, and Applications of Science

Engineering Design

HS-ETS1-2.

HS-ETS1-3.

HS-ETS1-4.

Earth and Space Sciences

HS-ESS3-3.

HS-ESS3-4.

Robert Rioux

Overview

Principles of Proper Waste Disposal

Procedure for Waste Handling

Summary

Threats to Biodiversity

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The Carbon Cycle

Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among ...

Energy Dynamics

The Food Chain
Energy is one of the most important abiotic factors in an ecosystem and organisms in an ecosystem are connected by the flow of energy and ...

What is Climate?

Climate refers to the prevailing weather conditions in a specific area over an extended period. As the saying goes, &#8220;Climate is what you expect. ...

Sustainable Development

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Global Climate Change

Throughout its ~4.5 billion year history, the Earth has experienced periods of warming and cooling. However, the current drastic increase in global ...

Extinction

The History of Life on Earth
Fossils of organisms that were different from anything alive today had been documented as early as the 17th century, but it ...

Ecological Disturbance

An ecological disturbance is a temporary disruption in the environment resulting from abiotic, biotic, or anthropogenic factors, causing a pronounced ...

Climate Change

The certainty of climate change remains a public controversy despite the consensus among approximately 97% of active climate researchers, who not only ...

Habitat Fragmentation

Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land ...

Analyze and interpret data to explain the impacts of land use on Earth's systems.

ESS.EES.5.1

water-quality-analysis-via-indicator-organisms

Water Quality Analysis via Indicator Organisms

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba -The University of Arizona
Demonstrating Author: Luisa Ikner
Water quality analysis monitors ...

Nutrients in Aquatic Ecosystems

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
Nitrogen and phosphorus are essential plant nutrients found in aquatic ...

turbidity-and-total-solids-in-surface-water

Turbidity and Total Solids in Surface Water

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
Turbidity and total solids are related measurements addressing clarity of ...

environmental-applications-of-microorganisms

Environmental Applications of Microorganisms

Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as ...

Dissolved Oxygen in Surface Water

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
Dissolved oxygen (DO) measurements calculate the amount of gaseous oxygen ...

The Water Cycle

The Earth&rsquo;s hydrosphere includes all of the areas where the storage and movement of water occurs. Since water is the basis of all living processes, ...

Biodiversity and Human Values

Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather ...

Analyze and interpret data to evaluate how human use of ground and surface waters impacts water quality and availability in river basins, wetlands, estuaries, and aquifers.

ESS.EES.5.2

Measuring Tropospheric Ozone

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
Ozone is a form of elemental oxygen (O3), a molecule of three oxygen atoms ...

determination-of-nox-in-automobile-exhaust-using-uv-vis-spectroscopy

Determination Of NOx in Automobile Exhaust Using UV-VIS Spectroscopy

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
In the troposphere, ozone is naturally formed when sunlight splits nitrogen ...

What is Weather?

Overview

Weather refers to the current state of atmospheric conditions at a specific time and place. Weather is temporary and is influenced by the ...

The Sulfur Cycle

Sulfur, an important element in the chemical makeup of proteins, is recycled through the atmosphere and aquatic and terrestrial environments. Found in the ...

design-example-sustainability-in-concrete-building

Design Example: Sustainability in Concrete Building

A construction firm specializing in concrete buildings constructed a sustainable commercial building.&#160;
The various sustainable practices incorporated ...

The Efficient Level of Pollution

Producing goods often results in pollution as a byproduct, but eliminating all pollution isn&#39;t practical. Doing so would mean halting the production ...

Construct an argument to evaluate the ways that human activities influence atmospheric composition.

ESS.EES.5.3

Proton Exchange Membrane Fuel Cells

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University
The United States consumes a large amount of energy &#8211; the current ...

Dye-sensitized Solar Cells

Source: Tamara M. Powers, Department of Chemistry, Texas A&#38;M University
Today&#39;s modern world requires the use of a large amount of energy. While ...

Energy

The universe is composed of matter in different forms, and all forms of matter contain energy.&#160; The different forms of energy on Earth originate from ...

Construct an argument to evaluate the benefits and trade-offs of using non-renewable or renewable energy sources for electricity production and transportation fuels.

ESS.EES.5.4

Green Marketing

Green marketing is about promoting products or services by highlighting their environmental advantages.
These products or services are produced and ...

The Efficient Amount of Recycling I

In an imaginary town, Greendale, residents carelessly dispose of recyclable plastics due to minimal additional trash pickup fees, leading to recyclable ...

The Efficient Amount of Recycling II

In the town of Greendale, tackling the recyclable plastic waste issue with price or quantity mechanisms, such as a disposal fee, quota, or disposable ...

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins ...

Construct an argument to evaluate potential solutions that will ensure sustainable consumption of Earth's resources.

Community Diversity

Populations do not live in isolation; thus, every population interacts with others in certain ways. These interactions give rise to a network of ...

Construct an argument to evaluate a range of solutions to mitigate impacts of human activities on Earth's systems.

ESS.EES.5.6

Evaluate how human consumption patterns impact Earth's systems.

Lab Waste Management and Safety

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