Haustral Churning

Haustral churning is a rhythmic mixing movement of the large intestine that helps process and compact its contents. Segmental contractions of the colon alternately tighten the circular muscles, causing material within neighboring haustra, or pouch-like sections, to move back and forth rather than rapidly advance. This motion exposes the intestinal contents to the mucosal surface, supporting absorption of water and electrolytes while promoting gradual formation of feces. Haustral churning therefore contributes to colonic motility, waste consolidation, and regulated transit before elimination, making it an important process for understanding digestive physiology and disorders involving altered bowel movement.

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JoVE Journal - Biology

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

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Cited by 22 •

2016

Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.

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