Villi Removal Technique
Villi Removal Technique - Related Videos
10:51
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
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Cited by 68 •
2016
We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.
04:06
Superior Lacrimal Gland Removal: A Surgical Technique to Remove the Palpebral and Orbital Parts of Superior Lacrimal Gland from Rabbit Model
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2025
This video describes the surgical procedure to remove the two parts: palpebral and orbital of the superior lacrimal gland from a rabbit to create a dry eye disease model. This rabbit model is suitable for studies of ocular surface homeostasis, pathophysiology and ocular therapeutics.
05:08
A Stereotactically Guided Ablation Technique for Auditory Cortex Removal in Rats
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2025
This video demonstrates a stereotactically-guided surgical procedure for ablating the auditory cortex in a rat. The steps include identifying anatomical landmarks in an anesthetized rat secured in a stereotactic frame, creating a window on the skull above the auditory cortex, aspirating the cortical layers, and providing post-operative care to assess the impact of the ablation on sound processing.
06:40
3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
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Cited by 2 •
2021
The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential.
05:42
Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
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Cited by 6 •
2022
The Fluorescence-aided Identification Technique is a practicable, fast, and reliable approach for the differentiation of composite resin restorations from tooth substance, and facilitates the minimally invasive and complete removal of composite resin restorations and composite bonded trauma splints.
FAQs
Selectivity depends on controlled mechanical disruption or careful tissue handling that detaches villus-associated cells without removing the deeper crypt region. This distinction matters because the crypt compartment contains proliferative and stem-like epithelial populations, whereas the villous surface represents a different epithelial context. Preserving both fractions allows investigators to compare compartment-specific biology rather than analyze a mixed tissue population.
Separating villous and crypt regions reduces the mixing of biologically distinct cell populations in the same sample. A less heterogeneous preparation can make molecular profiles, culture behavior, and histological features easier to attribute to a particular intestinal compartment. In cancer research, this improves comparisons between normal tissue and tumor-associated regions by limiting signals that could otherwise obscure compartment-specific differences.
The separated fractions support examination of epithelial biology across regions with different cellular characteristics. Researchers can focus on villus-associated cells or on crypt populations enriched for proliferative and stem-like epithelial cells, then assess their culture properties, molecular profiles, or histology. These comparisons are relevant to questions about epithelial regeneration and the cellular origins or development of intestinal tumors.
The workflow centers on handling intestinal tissue under controlled conditions, applying mechanical disruption or related tissue manipulation to detach the villous surface, and retaining the underlying crypt compartment. The resulting fractions can then be directed to culture, molecular profiling, or histological assessment. Maintaining separation during preparation is essential because the technique's value depends on preserving compartment-specific material for downstream analysis.
Material obtained through villus and crypt separation can support several complementary analyses. Researchers may culture the enriched cell populations, profile their molecular characteristics, or examine tissue organization histologically. Using these approaches together can connect cellular behavior with molecular and structural findings, helping investigators determine whether differences between intestinal compartments reflect normal epithelial biology, regeneration, or tumor-associated changes.
In colorectal cancer studies, compartment-specific samples can improve comparisons between normal intestinal tissue and tumor-associated compartments. Investigators can examine changes in epithelial populations, evaluate features linked to tumor initiation, and study how regeneration-related biology differs across regions. Enriching distinct cell populations also supports more focused interpretation of molecular or histological results than analysis of unsorted intestinal tissue.