Surgical Injection Technique
Surgical Injection Technique - Related Videos
07:34
Minimally-invasive Technique for Injection into Rat Optic Nerve
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Cited by 4 •
2015
Direct injection into the rat optic nerve is useful for regenerative research. We demonstrate a minimally-invasive technique for direct injection into a rat optic nerve that does not involve opening the skull. Using this method, surgical complications are minimized and recovery is more rapid.
06:08
A Standardized Surgical Technique for Tessier Medial Transnasal Canthopexy
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2026
Medial canthopexy allows correction of the position and fixation of the medial canthal tendon. Due to the technical difficulties of this procedure, there is a need for a detailed protocol for educational purposes. Here, we describe a standardized reproducible technique for bilateral medial transnasal canthopexy by an orbitonasal approach.
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
09:23
Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs
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Cited by 24 •
2012
Short visual description of the surgical technique and device used for the delivery of (gene and cell) therapies into the spinal cord. The technique is demonstrated in the animal but is entirely translatable and currently being used for human application.
07:54
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
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Cited by 15 •
2015
Stereotactic injection of lentiviruses expressing cDNAs or shRNAs can modulate gene expression in specific brain areas of mice. Here, we present a protocol to combine stereotactic injections with behavioral tasks, such as the Open Field Test (OFT) and the Forced Swim Test (FST).
FAQs
Depth determines which tissue region receives the material, while volume controls how much is released at that location. Selecting both parameters allows researchers to adjust local exposure and reduce unintended distribution into nearby or distant tissues. In cancer studies, this control helps distinguish effects caused by treatment at the intended site from effects related to broader material dispersal.
Anatomical targeting links the delivered material to a defined tissue or tumor location. This relationship is important because researchers may need to evaluate local drug activity, tumor behavior, or biological responses at the treated site. Accurate placement also helps limit exposure outside the intended region, making procedure-related effects easier to interpret.
The needle or catheter provides the route for releasing a measured amount of material, while operative guidance helps direct that instrument toward the selected anatomical site. Together, these components support controlled placement and depth selection. Their coordinated use is especially relevant when researchers need localized delivery during an operation rather than broad distribution throughout the body.
Researchers can use the technique to place a therapeutic agent directly within a tumor, creating localized exposure for treatment-response studies. This approach allows investigators to examine how the tumor responds at the treated site while limiting distribution to surrounding or distant tissues. The resulting observations can contribute to evaluations of drug activity and tumor biology.
A biological sample, including tumor cells, can be introduced into a selected anatomical site to support implantation in an animal model. Controlled placement helps associate later observations with the intended site of tumor development. Researchers can then use the model to study tumor biology, assess experimental interventions, and examine effects associated with the injection procedure.
Delivery of therapeutic agents, gene-based or immune-based therapies, and imaging agents can generate different types of cancer research information. Therapeutic materials support treatment-response evaluation, biological agents help investigate tumor-related processes, and imaging agents assist with observing relevant features. Researchers may also assess procedure-related effects alongside tumor biology and response outcomes.