Surgical Lesion Model
Surgical Lesion Model - Related Videos
10:09
Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease
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Cited by 121 •
2012
A protocol for performing unilateral 6-OHDA lesions of the medial forebrain bundle in mice is described. This method has a low mortality rate (13.3 %) with 89% of the surviving animals showing >95% loss of striatal dopamine and 90.63±-4.02 % ipsiversive rotational bias towards the side of the lesion.
05:51
A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
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Cited by 13 •
2014
Atherosclerosis is a chronic inflammatory process. This manuscript illustrates an easy to use ex vivo model to investigate fresh carotid or coronary artery plaques. The ex vivo model allows for the investigation of potential substances on the inflammatory milieu in human atherosclerotic lesions and results can be analyzed by various methods.
08:02
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
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Cited by 62 •
2012
A description of the surgical induction of endometriosis in mice and rats by auto-transplantation of uterine tissue to the arterial cascade of the intestinal mesentery.
08:06
Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test
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Cited by 57 •
2010
Parkinson disease is caused by loss of dopaminergic innervation to the striatum, which can be experimentally induced by 6-OH-dopamine. We describe how to perform a stereotaxic lesion and to monitor apomorphine-induced rotational behavior in mice. This model is useful and reliable for testing new therapies for Parkinson disease.
08:40
Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies
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Cited by 226 •
2013
Photothrombosis is a quick, minimally-invasive technique for inducing small and well-delimited infarction in areas of interest in highly reproducible manner. It is particularly suitable for studying cellular and molecular responses underlying brain plasticity in transgenic mice.
FAQs
Local tissue interactions help determine how neighboring cells respond when a region is excised or disrupted. The lesion changes the nearby signaling environment, so cells may alter movement, wound responses, or differentiation. Observing these changes links a physical disturbance to developmental patterning mechanisms and shows how local communication contributes to organ formation and restoration.
Changes in differentiation indicate whether surrounding cells retain flexible developmental potential after injury. If cells adopt different fates or contribute to restoring a missing structure, the response can reveal how developmental fields coordinate pattern and organ formation. This makes differentiation a useful outcome for connecting local damage with broader developmental plasticity.
The unlesioned tissue provides a reference for normal structure and developmental behavior, while the lesioned tissue shows the consequences of controlled disruption. Comparing the two helps distinguish wound responses, altered tissue movement, or regeneration from ordinary development. This contrast provides direct evidence about the roles of tissue interactions in pattern establishment and structural restoration.
A missing or disrupted region can show whether developmental mechanisms continue operating during repair. Researchers can examine how cells respond to the wound, reorganize locally, differentiate, and potentially restore absent structures. These observations connect regeneration with developmental patterning rather than treating repair as an isolated process, highlighting shared roles for tissue interactions and signaling environments.
A typical study creates a defined tissue excision or disruption through microsurgery, maintains the experimental system under controlled conditions, and then observes the affected region over the relevant developmental response. Researchers assess wound responses, tissue movement, differentiation, and restoration, using an unlesioned comparison when appropriate to interpret changes against normal development.
Researchers can document how the wound responds, whether nearby tissues move into or around the affected area, and whether differentiation changes after the intervention. They can also assess restoration of missing structures and compare these outcomes with unlesioned tissue. Together, these observations reveal how developmental fields coordinate pattern, organ formation, and repair.
These models help investigate congenital defects, repair processes, and developmental plasticity by deliberately altering local tissue relationships. They are especially useful when researchers need direct evidence for how tissue interactions influence pattern formation or organ development. Lesion-based comparisons can also clarify whether an organism restores structure through coordinated developmental and regenerative responses.