Mycobacterium Marinum
Mycobacterium Marinum - Related Videos
07:00
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
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Cited by 19 •
2018
Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.
10:19
Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
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Cited by 25 •
2014
This video article describes the high throughput pipeline that has been successfully established to infect and analyze large numbers of zebrafish embryos providing a new powerful tool for compound testing and drug discovery using a whole animal vertebrate organism.
09:03
Growth of Mycobacterium tuberculosis Biofilms
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Cited by 79 •
2012
Mycobacterium tuberculosis forms drug tolerant biofilms when cultured in certain conditions. Here we describe methods for culturing M. tuberculosis biofilms and determining the frequency of drug tolerant persisters. These protocols will be useful for further studies into the mechanisms of drug tolerance in M. tuberculosis.
03:46
Isolation of Siderophore-Containing Extracellular Vesicles from Mycobacterium tuberculosis
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2025
Source: Gupta, S., Marcela Rodriguez, G. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (2019)This video demonstrates the stepwise isolation of extracellular vesicles (EVs) from Mycobacterium tuberculosis cultured under iron-limiting conditions that mimic the host environment. It outlines the use of ultrafiltration, differential centrifugation, and density gradient separation to enrich and identify EV-containing fractions.
04:06
Preparation and Quantification of Mycobacterium bovis BCG lux Inoculum for Infection
0 Views •
2025
This video demonstrates the preparation and quantification of Mycobacterium bovis Bacillus Calmette-Guérin lux inoculum using bioluminescence assay and the enumeration of colony-forming units. The prepared inoculum can be used for infection studies.
FAQs
Persistence within macrophages allows M. marinum to remain inside host immune cells rather than being cleared immediately. This intracellular setting gives researchers a way to examine how a mycobacterial pathogen interacts with macrophage defenses and how infection can continue while immune responses develop. The resulting observations are relevant to understanding host-pathogen interactions in immunology and infection research.
Granuloma formation provides a visible indication of organized host inflammation around mycobacterial infection. Studying these structures helps researchers investigate how immune cells respond to persistent organisms and how inflammation develops over time. Because M. marinum produces tuberculosis-like disease in fish and parallels aspects of M. tuberculosis infection, granuloma biology is especially useful for connecting experimental findings with broader mycobacterial pathogenesis.
M. marinum’s preference for cooler temperatures helps explain why human disease usually remains localized rather than becoming widespread throughout the body. Temperature therefore acts as an important biological condition when interpreting infection behavior. This feature also helps researchers relate the organism’s environmental setting to the sites and patterns in which infection develops after aquatic exposure.
M. marinum provides a tractable system for examining processes that are difficult to study directly in M. tuberculosis, including mycobacterial entry, intracellular survival, host inflammation, and granuloma biology. Its close parallels with M. tuberculosis allow researchers to investigate selected features of virulence and antimicrobial responses while focusing on experimentally accessible infection processes.
Aquatic exposure, damaged skin, and temperature are central conditions for interpreting M. marinum infection. Connecting these factors with the later appearance of slowly developing nodules or ulcers helps researchers relate the route and environment of exposure to disease presentation. This framework is particularly relevant when analyzing why infection typically remains localized in human cases.
M. marinum models can provide information about how mycobacteria enter host tissues, persist within macrophages, stimulate inflammation, and contribute to granuloma formation. They also support investigations of virulence and antimicrobial responses. Together, these outcomes connect cellular mechanisms with tissue-level disease and make the organism useful for studying both host defenses and infection progression.