Establishment and Optimization of a High Throughput Setup to Study <em>Staphylococcus epidermidis</em> and <em>Mycobacterium marinum</em> Infection as a Model for Drug Discovery

Wouter J. Veneman; Rub&#233;n Mar&#237;n-Juez; Jan de Sonneville; Anita Ordas; Susanne Jong-Raadsen; Annemarie H. Meijer; Herman P. Spaink

doi:10.3791/51649

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Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Establishment and Optimization of a High Throughput Setup to Study <em>Staphylococcus epidermidis</em> and <em>Mycobacterium marinum</em> Infection as a Model for Drug Discovery

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

13,120 Views

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10:19 min

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June 26, 2014

DOI:

10.3791/51649-v

DOI:

10.3791/51649-v

•

10:19 min

June 26, 2014

•

3 Views

Wouter J. Veneman*¹, Rubén Marín-Juez*², Jan de Sonneville³, Anita Ordas¹, Susanne Jong-Raadsen², Annemarie H. Meijer¹, Herman P. Spaink¹

¹Institute of Biology,Leiden University, ²ZF-screens BV, ³Life Science Methods BV

Summary

This video article describes the high throughput pipeline that has been successfully established to infect and analyze large numbers of zebrafish embryos providing a new powerful tool for compound testing and drug discovery using a whole animal vertebrate organism.