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Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery

DOI:
10.3791/51649-v

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10:19 min

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June 26, 2014

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, , , , , ,
1Institute of Biology,Leiden University, 2ZF-screens BV, 3Life Science Methods BV

Chapters

  • 00:05Title
  • 01:17Prepare Bacterial Strains and Collect Zebrafish Eggs
  • 03:45Embryo Injections
  • 05:32Flow Cytometry
  • 07:14High Resolution Imaging
  • 08:07Results: Analysis of Bacterial Injection in Zebrafish Embryos
  • 09:51Conclusion

Summary

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Automatic Translation

This video article describes the high throughput pipeline that has been successfully established to infect and analyze large numbers of zebrafish embryos providing a new powerful tool for compound testing and drug discovery using a whole animal vertebrate organism.

Tags

Keywords: ZebrafishHigh ThroughputDrug DiscoveryStaphylococcus EpidermidisMycobacterium MarinumInfection ModelPreclinical ScreeningIn Vivo ModelTransgenic Lines

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