Scintillation Counter
Scintillation Counter - Related Videos
10:52
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Cited by 21 •
2016
Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with 3H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Cited by 18 •
2012
The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
07:31
Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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2024
A protocol is described for preparing a simple model ecosystem that recreates the methane-oxygen counter gradient found in the natural habitat of aerobic methane-oxidizing bacteria, enabling the study of their physiology in a spatially resolved context. Modifications to common biochemical assays for use with the agarose-based model ecosystem are also described.
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
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Cited by 2 •
2010
This procedure describes a quick and easy workflow to introduce siRNA into difficult to transfect cell lines and follow gene expression by real-time PCR. Use of an automated cell counter, multi-well electroporation plate, and automated electrophoresis station provide quick and reliable results without the need for expensive robotic handling.
FAQs
Each radiation event deposits energy in the scintillator, producing a flash whose light output is converted by a photomultiplier tube or another photodetector into an electrical pulse. The instrument can then register separate events and assess their pulse characteristics. This conversion creates a measurable link between radioactive decay and quantitative assay results.
The number of electrical pulses reflects how many radiation events the instrument detects, whereas pulse intensity reflects the characteristics of the light flash generated after energy deposition. Together, these signals indicate radioactive activity and support quantitative interpretation. Separating event frequency from signal strength helps researchers extract more information than a simple visual measurement could provide.
The scintillator provides the light-producing step after radiation deposits energy, while the photomultiplier tube or other photodetector performs the light-to-electrical conversion needed for counting. Neither stage alone supplies the complete measurement pathway. Their sequential roles allow radiation that cannot be directly assessed by ordinary visual observation to become an analyzable electrical signal.
Radiolabeled molecules can participate in biological processes while their associated radiation remains measurable. A scintillation counter detects the resulting events with high sensitivity and converts them into quantitative activity data. This capability supports measurements of tracer uptake, antigen-antibody binding, and cell proliferation, allowing researchers to compare biological responses through recorded radioactive signals.
Researchers first use a radiolabeled molecule in an assay designed to examine uptake, binding, or proliferation. The resulting radioactive signal is then measured by the counter as detected events and pulse characteristics. Those measurements provide quantitative data for interpreting the biological process under study, such as immune-cell activity or antigen-antibody interaction.
In immunology, the method can quantify antigen-antibody binding, tracer uptake, or cell proliferation, all of which reflect different aspects of an immune response. In infection research, the same measurements can help follow pathogen-related processes. The resulting activity data connect radiolabeled assay signals with changes in cellular or molecular behavior.
Because the instrument produces quantitative measurements from radiolabeled assays, researchers can use changes in detected activity to evaluate experimental treatments. Depending on the assay, the measured outcome may involve tracer uptake, antigen-antibody binding, cell proliferation, or a pathogen-related process. Comparing these signals helps characterize whether treatment conditions alter the biological response being studied.