Surface Marker Antibodies
Surface Marker Antibodies - Related Videos
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
11:56
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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Cited by 24 •
2014
We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.
09:38
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
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Cited by 38 •
2011
Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.
FAQs
Labeling depends on the match between an antibody’s binding site and an epitope, the specific molecular feature recognized on a cell-surface protein or carbohydrate. Because different cell populations display different surface molecules, this interaction provides the basis for selective identification. The resulting specificity allows researchers to distinguish related populations rather than treating all cells in a sample as equivalent.
The antibody provides molecular recognition, while the attached tag supplies a detectable signal. Fluorophores support fluorescence-based measurements such as flow cytometry or immunofluorescence, whereas enzymes and other tags enable related detection formats. This separation of recognition from readout lets the same binding principle be adapted to different assays and makes labeled cell populations measurable or visible.
A single marker can help identify a population, but patterns across several markers provide information about distinctions within that population. In immunology research, these patterns support characterization of immune-cell subsets and assessment of activation or differentiation. Comparing marker profiles can therefore reveal changes in cellular composition and state after infection, during disease, or following treatment.
A typical workflow begins by selecting antibodies directed against the surface features relevant to the cell populations under study. The antibodies are allowed to bind their target epitopes, and the attached fluorophore, enzyme, or other tag is then detected with a compatible assay. Researchers interpret the resulting signal to identify, classify, or quantify labeled populations.
The choice depends on the desired form of information. Flow cytometry uses detectable antibody tags to analyze labeled cells and quantify cellular responses or population changes, while immunofluorescence makes bound markers observable in a fluorescence-based assay. Both approaches use selective surface binding, but they support different ways of examining cell populations and their marker patterns.
In infection research, these reagents help distinguish immune-cell subsets, identify infected or otherwise altered cells, and monitor how pathogens influence cellular composition and function. The same measurements can be used to evaluate treatment-associated changes. By comparing marker-defined populations and their signals, researchers can characterize disease-associated responses and assess shifts in immune organization.