Lollipop Ultrafiltration Probe
Lollipop Ultrafiltration Probe - Related Videos
08:37
Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry
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Cited by 2 •
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Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.
02:49
Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
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This video demonstrates the isolation and concentration of bacterial culture filtrate proteins, which include bacterial extracellular vesicles, using ultrafiltration. The concentrated extracellular vesicles serve as diagnostic markers for disease detection.
10:19
Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
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Cited by 9 •
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Design and fabrication of a three-dimensionally (3-D) printed microfluidic cross-flow filtration system is demonstrated. The system is used to test performance and observe fouling of ultrafiltration and nanofiltration (thin film composite) membranes.
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
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Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
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This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
FAQs
Selectivity comes from the probe’s semipermeable membrane. Under pressure, interstitial fluid crosses it, allowing water and small dissolved molecules to enter the collection pathway, while cells and larger components remain outside. This size-based separation produces an ultrafiltrate suitable for chemical analysis and reflects locally available small solutes without including intact cellular material.
Pressure is the driving condition that moves interstitial fluid across the membrane and into the collection route. The recovered volume and chemical content therefore depend on fluid being pushed through this selective interface. Because the membrane retains cells and larger components, the sample represents a filtered fraction of the local environment rather than a complete specimen.
The recovered ultrafiltrate can be examined for metabolites, drugs, electrolytes, and disease-related biomarkers. These targets give the probe several analytical roles: it can characterize local chemistry, follow the presence of administered or endogenous substances, and assess molecular changes associated with disease. Such measurements connect tissue-level conditions with chemical readouts.
A sampling workflow begins with positioning the rounded probe head in the tissue or biological environment of interest. Pressure then drives local interstitial fluid through the membrane, and the resulting ultrafiltrate travels into the collection pathway. The collected sample is subsequently subjected to chemical analysis. This sequence links a local sampling site to measurable molecular information.
Localized sampling links chemical measurements to the physiology of the specific tissue or biological environment being examined. Because the probe can provide time-resolved measurements, investigators can track changing local conditions rather than relying on a single chemical snapshot. This supports studies of treatment responses and may clarify how disease-related biomarkers vary at the sampling site.
In medicine, investigators can use this approach when they need chemical information from a particular tissue or biological environment through a minimally invasive sampling strategy. It supports examination of treatment responses, changing tissue conditions, and disease-related biomarker patterns, keeping the research focus on local physiology.