Luminal Ph Profiling
Luminal Ph Profiling - Related Videos
08:24
Profiling Luminal pH in Three-Dimensional Gastrointestinal Organoids Using Microelectrodes
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Cited by 2 •
2024
The present protocol describes pH measurements in human tissue-derived gastric organoids using microelectrodes for spatiotemporal characterization of intraluminal physiology.
10:21
Studying Murine Small Bowel Mechanosensing of Luminal Particulates
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Cited by 3 •
2022
To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
09:17
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
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Cited by 1 •
2022
This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.
FAQs
At each measurement point, pH-sensitive electrodes or ingestible sensors detect hydrogen-ion activity. Recording values at defined locations and time points allows investigators to distinguish a change confined to one region from a pattern that evolves over time. The resulting profile therefore links luminal chemistry with anatomical location and temporal behavior, rather than providing only a single acidity reading.
A pH profile can quantify acid exposure by showing how strongly and how long luminal conditions shift toward acidity. It can also reveal abnormal buffering, meaning altered neutralization, and transit-related patterns as chemistry changes across regions or time. These distinctions help characterize physiology and disease beyond a single isolated measurement.
Regional and temporal views answer different questions. Regional comparisons show where luminal chemistry differs within the hollow organ, while repeated time-point measurements show whether those differences persist, fluctuate, or shift. Considering both dimensions can expose abnormal buffering or transit patterns that a single location or isolated time point might miss, improving physiological and disease characterization.
Measurement planning starts with choosing a pH-sensitive electrode or ingestible sensor appropriate to the intended observation. Investigators then define the anatomical locations and time points needed to address the study question. Organizing readings this way produces a regional and temporal profile that can be compared with physiological or disease-related patterns.
In clinical medicine, measurements can support evaluation of reflux and other disorders involving luminal chemistry. The key outcome is not merely a pH value, but the distribution and timing of acid exposure and related changes across the measured space. That profile can help characterize abnormal physiology and provide a basis for assessing disease-related patterns.
In research, profiles can be used to examine digestion, assess responses to treatment, and determine how medications or dietary factors alter the gastrointestinal environment. Comparing profiles across these conditions can show whether luminal chemistry changes regionally or over time. This makes the technique useful for linking an intervention or exposure with measurable gastrointestinal changes.