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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Article DOI: 10.3791/1773-v • 12:23 min • May 25th, 2010
May 25th, 2010 •
*1,2, *1, 1,3,4, 1,2,5,6,7
1Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering, McGill University, 3Program in Neuroengineering, McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology, McGill University, 6Department of Biology, McGill University, 7Department of Medicine, Universite de Montreal - University of Montreal
* These authors contributed equally

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This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

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Dissection Culture Commissural Neurons Embryonic Spinal Cord Axon Guidance Dorsal Spinal Cord Stereotyped Trajectories Guidance Cues Turning Assays Immunochemistry Biochemistry E13 Rat Dissociation Cell Suspension Poly-L-lysine-coated Glass Coverslips Tissue-culture Dishes In Vitro Studies Cellular Mechanisms Molecular Mechanisms
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