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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Article DOI: 10.3791/4378-v • 09:19 min • October 19th, 2012
October 19th, 2012 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine

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We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.

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SNARE-mediated Membrane Fusion Enzymatic Cell Fusion Assay SNARE Proteins V-SNAREs T-SNAREs Intracellular Vesicle Fusion Reconstitution Assays Cell Fusion Assay Flipped SNARE Proteins Microscopic Analysis Quantification Of Cell Fusion Events β-galactosidase Tet-Off Gene Expression System Tetracycline-controlled Transactivator (tTA) Reporter Plasmid LacZ Gene Tetracycline-response Element (TRE-LacZ) COS-7 Cells
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