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RNA-seq Analyse Transkriptomen Thrombin-behandelt und Control Human Pulmonary mikrovaskulären Endothelzellen
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RNA-seq Analyse Transkriptomen Thrombin-behandelt und Control Human Pulmonary mikrovaskulären Endothelzellen

Article DOI: 10.3791/4393-v • 18:30 min • February 13th, 2013
February 13th, 2013 •
1, 1, 1, 1, 1, 1, 1
1Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City

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Dieses Protokoll stellt eine vollständige und detaillierte Verfahren zur RNA-seq, eine leistungsstarke Next-Generation-DNA-Sequenzierung Technologie zum Profil Transkriptomen in der menschlichen Lunge mikrovaskulären Endothelzellen mit oder ohne Thrombin Behandlung anzuwenden. Dieses Protokoll ist generalisierbar verschiedenen Zellen oder Geweben von verschiedenen Reagenzien oder Krankheitszuständen befallen.

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Genetik Molekularbiologie Immunologie Medizin Genomics Proteine RNA-seq Next Generation DNA-Sequenzierung Transkriptom Transkription Thrombin Endothelzellen High-Throughput- DNA genomische DNA RT-PCR PCR
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