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JoVE Journal Developmental Biology
Visualisering av kondrocyttransplantation interkalering och riktnings spridning via Zebrabow Kloncellanalys i embryonala Meckels Brosk
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Visualisering av kondrocyttransplantation interkalering och riktnings spridning via Zebrabow Kloncellanalys i embryonala Meckels Brosk

Article DOI: 10.3791/52935-v • 06:40 min • October 21st, 2015
October 21st, 2015 •
*1, *1, 1, 1
1Massachusetts General Hospital, Center for Regenerative Medicine, Harvard Medical School
* These authors contributed equally

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Cell organisation av kraniofaciala ben har länge varit en hypotes men aldrig direkt visualiseras. Multi-spektral cellmärkning och in vivo levande avbildning möjliggör visualisering av dynamiska cellbeteende i zebrafisk käken. Här, att vi i detalj protokollet manipulera Zebrabow transgen fisk och direkt observera cell inlagring och morfologiska förändringar av kondrocyter i Meckels brosk.

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Utvecklingsbiologi Kraniofaciala utveckling hjärn neurallist konfokalmikroskopi öde kartläggning multi-spektrala klonal analys sox10 Zebrabow zebrafisk underkäken Meckels brosk
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