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JoVE Journal Developmental Biology
Lichtvermittelte reversible Modulation des mitogenaktivierten Proteinkinase-Pathways während der Zelldifferenzierung und
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Lichtvermittelte reversible Modulation des mitogenaktivierten Proteinkinase-Pathways während der Zelldifferenzierung und

Article DOI: 10.3791/55823-v • 09:32 min • June 15th, 2017
June 15th, 2017 •
*1, *2, 1, 1, 1, 2, 2, 1,3,4
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 3Neuroscience Program, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Dieses Protokoll beschreibt eine optogenetische Strategie zur Modulation der mitogenaktivierten Proteinkinase (MAPK) Aktivität während der Zelldifferenzierung und der Xenopus embryonalen Entwicklung. Diese Methode ermöglicht die reversible Aktivierung des MAPK-Signalwegs in der Säugerzellkultur und in multizellulären Lebendorganismen wie Xenopus- Embryonen mit hoher räumlicher und zeitlicher Auflösung.

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Entwicklungsbiologie Ausgabe 124 Optogenetik Protein-Protein-Wechselwirkungen PC12-Zelldifferenzierung, Embryonale Entwicklung Kryptochrom CRY2-CIBN bicistronic Raf / MEK / ERK Kinase Signalisierung Kaskade
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