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JoVE Journal Developmental Biology
En RANKL-baserede osteoklast kultur Assay af musen knoglemarven til at undersøge rollen, som mTORC1 i osteoklast dannelse
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En RANKL-baserede osteoklast kultur Assay af musen knoglemarven til at undersøge rollen, som mTORC1 i osteoklast dannelse

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Dette manuskript beskriver en protokol til at isolere og kultur osteoklaster in vitro- fra mus knoglemarven og studere rollen til pattedyr/mekanistiske målet på rapamycin komplekse 1 i osteoklast dannelse.

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Udviklingsmæssige biologi sag 133 osteoklast mTORC1 Raptor ben remolding cellebiologi RANKL M-CSF
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