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JoVE Journal Developmental Biology
Generasjon av stillaset-fri, tredimensjonale Insulin uttrykke Pancreatoids fra musen bukspyttkjertelen Progenitors In Vitro
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Generasjon av stillaset-fri, tredimensjonale Insulin uttrykke Pancreatoids fra musen bukspyttkjertelen Progenitors In Vitro

Article DOI: 10.3791/57599-v • 09:33 min • June 2nd, 2018
June 2nd, 2018 •
1, 2,3,4, 2,3,4, 1,2,3,4,5
1Program in Developmental Biology, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children's Hospital, and Houston Methodist Hospital, Baylor College of Medicine, 3Molecular and Cellular Biology Department, Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center, Baylor College of Medicine, 5McNair Medical Institute, Baylor College of Medicine

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Her presenterer vi en protokoll for å generere insulin uttrykke 3D murine pancreatoids fra frittflytende e10.5 dissosiert bukspyttkjertelen progenitors og den tilknyttede mesenchyme.

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Utviklingsbiologi problemet 136 bukspyttkjertelen Organoids Beta-cellene utvikling Mesenchyme differensiering Diabetes 3D kultur Pancreatoids
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