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Isolation, karakterisering og High Throughput ekstracellulære Flux analyse af musen primær Renal tubulær epitelceller
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Isolation, karakterisering og High Throughput ekstracellulære Flux analyse af musen primær Renal tubulær epitelceller

Article DOI: 10.3791/57718-v • 09:40 min • June 20th, 2018
June 20th, 2018 •
1,2, 1,2, 2,3,4,5
1Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

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Denne protokol giver en omkostningseffektiv tilgang for at isolere og karakterisere mus primær renal tubulær celler, der kan efterfølgende sub kulturperler for at vurdere renal biologiske funktioner ex vivo, herunder mitokondrie bioenergetik.

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Biologi sag 136 primære celle isolation primære celle karakterisering rørformede epitelceller nyre bioenergetik mitokondrier respiration høj overførselshastighed ekstracellulære flux analyse fedtsyre oxidation kronisk nyresygdom
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