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Gebruik van één molecuul fluorescentie In Situ hybridisatie (SM-vis) om Quantify en lokaliseer mRNAs in lymfkliertest eicellen

Article DOI: 10.3791/59414-v • 08:18 min • April 24th, 2019
April 24th, 2019 •
1, 1
1Department of Animal Science, University of Nebraska-Lincoln

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Reproducibly het om getallen te tellen van mRNAs in individuele eicellen, enkel molecuul RNA fluorescentie in situ is hybridisatie (RNA-vis) geoptimaliseerd voor niet-aanhanger cellen. Eicellen werden verzameld, gekruist met het transcript specifieke sondes en gekwantificeerd aan de hand van een afbeelding kwantificering software.

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Genetica kwestie 146 oöcyt mRNA kwantificering mRNA lokalisatie niet-aanhanger cellen fluorescentie in situ hybridisatie vertakte DNA
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