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In Vivo Avanti Schermo Genetico per Identificare Nuovi Geni Neuroprotettivi in Drosophila melanogaster

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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Vi presentiamo un protocollo utilizzando un approccio genetico in avanti allo screening per i mutanti che mostrano neurodegenerazione in Drosophila melanogaster. Incorpora un saggio di arrampicata, analisi istologica, mappatura genica e sequenziamento del DNA per identificare in ultima analisi nuovi geni legati al processo di neuroprotezione.

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Genetica Numero 149 Drosophila melanogaster neurodegenerazione schermo genetico avanzato analisi di arrampicata istologia mappatura genica sequenziamento del DNA
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