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Mätning relativ insulinsekretion med hjälp av en co-utsöndras Luciferase surrogat
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Mätning relativ insulinsekretion med hjälp av en co-utsöndras Luciferase surrogat

Article DOI: 10.3791/59926-v • 05:58 min • June 25th, 2019
June 25th, 2019 •
1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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Detta protokoll beskriver hur man utför snabb låg kostnad luciferas analyser på medel lång genom strömning med hjälp av en insulin-länkade Gaussia luciferas som en proxy för insulinsekretion från beta-celler. Analysen kan utföras med de flesta luminiscens plattan läsare och Multichannel pipetter.

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Biologi insulin utsöndras luciferas bukspottskörteln beta cell diazoxid paradigm sekretion assay Gaussia
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