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Isolamento delle cellule CD8 + T residenti neireni del topo per l'analisi della citometria a flusso

Article DOI: 10.3791/61559-v • 06:07 min • June 27th, 2020
June 27th, 2020 •
1,2, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

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A seguito di infezione virale, il rene ospita un numero relativamente elevato di cellule CD8+ T e offre l'opportunità di studiare le cellule T RM nonmucose. Qui descriviamo un protocollo per isolare i linfociti renali del topo per l'analisi della citometria del flusso.

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Questo mese in JoVE Numero 160 CD8 residente nei reni LCMV citometria a flusso mouse etichettatura intravascolare
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